Abstract

Human hepatitis B virus (HBV) belongs to a family of hepadnaviruses which replicate via the reverse transcription of an RNA intermediate, the pregenome. HBV infection represents a major worldwide human health problem. It causes acute, chronic hepatitis and the infection has been linked with hepatocellular carcinoma (HCC) in humans. An understanding of the biology of HBV has been severely limited due to its narrow host range and the inability to propagate in in vitro. The earliest consequence of HBV infection is the occurrence of acute phase of viral hepatitis B. This results in elevated levels of liver enzymes. In this study, our basic inquiry relates to whether HBV X gene product is responsible for the transcriptional activation of acute phase genes of the liver. Studies are proposed here to investigate in detail the mechanism of acute viral hepatitis resulting from HBV infections. In this pilot feasibility project we propose to study the acute-phase of viral hepatitis by investigating the role HBV genes, with particular emphasis on X gene product in the transcriptional activation of acute-phase genes in the liver.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
2P30DK034914-06
Application #
3854878
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
University of Colorado Denver
Department
Type
DUNS #
065391526
City
Aurora
State
CO
Country
United States
Zip Code
80045

  Publications

Sokol, Ronald J; Mack, Cara; Narkewicz, Michael R et al. (2003) Pathogenesis and outcome of biliary atresia: current concepts. J Pediatr Gastroenterol Nutr 37:4-21
Sokol, R J; Straka, M S; Dahl, R et al. (2001) Role of oxidant stress in the permeability transition induced in rat hepatic mitochondria by hydrophobic bile acids. Pediatr Res 49:519-31
Leslie, K K; Reznikov, L; Simon, F R et al. (2000) Estrogens in intrahepatic cholestasis of pregnancy. Obstet Gynecol 95:372-6
Simon, F R; Fortune, J; Iwahashi, M et al. (1999) Characterization of the mechanisms involved in the gender differences in hepatic taurocholate uptake. Am J Physiol 276:G556-65
Ostrowska, A; Karrer, F M; Bilir, B M (1999) Histological identification of purified and cryopreserved allogeneic hepatocytes following transplantation in a murine model without host immunosuppression. Transpl Int 12:188-94
Martin, S L; Maniero, G D; Carey, C et al. (1999) Reversible depression of oxygen consumption in isolated liver mitochondria during hibernation. Physiol Biochem Zool 72:255-64
Shivaram, K N; Winklhofer-Roob, B M; Straka, M S et al. (1998) The effect of idebenone, a coenzyme Q analogue, on hydrophobic bile acid toxicity to isolated rat hepatocytes and hepatic mitochondria. Free Radic Biol Med 25:480-92
Sokol, R J; McKim Jr, J M; Goff, M C et al. (1998) Vitamin E reduces oxidant injury to mitochondria and the hepatotoxicity of taurochenodeoxycholic acid in the rat. Gastroenterology 114:164-74
Podolin, D A; Sutherland, E; Iwahashi, M et al. (1998) A high-sucrose diet alters the lipid composition and fluidity of liver sinusoidal membranes. Horm Metab Res 30:195-9
Sokol, R J; Devereaux, M W; Khandwala, R (1998) Effect of oxypurinol, a xanthine oxidase inhibitor, on hepatic injury in the bile duct-ligated rat. Pediatr Res 44:397-401

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