Abstract

We propose to develop a novel computer-based system for performing simultaneous video imaging and patch-clamp recording from single cells and couplets. Although systems exist for the individual functions of fluorescence imaging, patch-clamp current recording and capacitance measurement, the synchronization and integration of these measurements in one experimental system has not been accomplished. We propose to develop such a system in three stages. In this proposal we request funding for the first two stages, which involve the design and implementation of the software that will control and synchronize the data collection and analysis for experiments: 1. Software design and patch-clamp interface. In the first year we intend to plan the architecture of the software, and to adapt as existing Macintosh-based patch-clamp acquisition program to work with the QuickTime synchronization system. 2. Implementation of quantitative image analysis. Work in the second year will be the adaptation of modules that perform fluorescence ratio and morphometric image processing to work in the QuickTime framework. These software developments form the basis for the third stage, the construction of the actual experimental setup.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
3P30DK034989-15S1
Application #
6105288
Study Section
Project Start
1998-09-01
Project End
1999-08-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
15
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Yale University
Department
Type
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code
06520

  Publications

Garcia-Tsao, Guadalupe (2018) Regression of HCV cirrhosis: Time will tell. Hepatology 67:1651-1653
Hung, Adelina; Garcia-Tsao, Guadalupe (2018) Acute kidney injury, but not sepsis, is associated with higher procedure-related bleeding in patients with decompensated cirrhosis. Liver Int 38:1437-1441
Kaffe, Eleanna; Fiorotto, Romina; Pellegrino, Francesca et al. (2018) ?-Catenin and interleukin-1?-dependent chemokine (C-X-C motif) ligand 10 production drives progression of disease in a mouse model of congenital hepatic fibrosis. Hepatology 67:1903-1919
Goldberg, David S; Levy, Cynthia; Yimam, Kidist et al. (2018) Primary Sclerosing Cholangitis Is Not Rare Among Blacks in a Multicenter North American Consortium. Clin Gastroenterol Hepatol 16:591-593
Cadamuro, Massimiliano; Stecca, Tommaso; Brivio, Simone et al. (2018) The deleterious interplay between tumor epithelia and stroma in cholangiocarcinoma. Biochim Biophys Acta Mol Basis Dis 1864:1435-1443
Besse, Whitney; Choi, Jungmin; Ahram, Dina et al. (2018) A noncoding variant in GANAB explains isolated polycystic liver disease (PCLD) in a large family. Hum Mutat 39:378-382
Strazzabosco, Mario; Fiorotto, Romina; Cadamuro, Massimiliano et al. (2018) Pathophysiologic implications of innate immunity and autoinflammation in the biliary epithelium. Biochim Biophys Acta Mol Basis Dis 1864:1374-1379
Murugesan, Vagishwari; Liu, Jun; Yang, Ruhua et al. (2018) Validating glycoprotein non-metastatic melanoma B (gpNMB, osteoactivin), a new biomarker of Gaucher disease. Blood Cells Mol Dis 68:47-53
Sachar, Hamita; Pichetshote, Nipaporn; Nandigam, Kavitha et al. (2018) Continued midazolam versus diphenhydramine in difficult-to-sedate patients: a randomized double-blind trial. Gastrointest Endosc 87:1297-1303
Bhutta, A Q; Garcia-Tsao, G; Reddy, K R et al. (2018) Beta-blockers in hospitalised patients with cirrhosis and ascites: mortality and factors determining discontinuation and reinitiation. Aliment Pharmacol Ther 47:78-85

Showing the most recent 10 out of 763 publications