The primary objectives of the Analytic Core are to: ? provide access to high-quality, cost-effective laboratory testing for Affiliate Investigators (Als) ? provide consultation concerning laboratory testing ? develop, evaluate, and improve laboratory tests in human and animal specimens A secondary objective is to provide training for young investigators in method development and quality control. The Analytic Core of the NORC will continue to provide access to a wide array of comprehensive, state-of-the-art laboratory services for NORC Als in a highly cost-effective fashion. This is done by developing specialized tests performed directly by NORC technologists (direct services) and by facilitating laboratory services in various parts of the clinical laboratories in the Department of Laboratory Medicine and in other laboratories at the University of Washington (UW) that provide laboratory services (indirect services). By referring testing to various laboratories within the UW while handling logistics for those referrals, the Analytic Core provides comprehensive services in a manner that is as seamless as possible for Als, and broader in scope than would be cost-effective or possible for the Analytic Core working in isolation and performing only direct testing. The Core is a service facility, with the main mission to provide laboratory testing requested by Als. This involves responding to direct requests for assays as well as anticipating future needs and research directions. Faculty and technologists frequently consult with Als, direct research, and train young investigators using Analytic Core facilities. As has occurred during the past several years, the Core has adapted to the needs of the Als, has incorporated and is continuing to adapt to new technologies, and performs developmental research to set up and/or facilitate access to analyses. Training opportunities have helped two of our trainees advance into junior faculty positions where they continue basic science research in nutrition and inflammation. During the current funding cycle, a major focus ofthe Analytic Core has been development of novel targeted assays that use liquid chromatography-tandem mass spectrometry (LC-MS/MS). These efforts were mounted in response to the changing needs of the Als and have supported large studies in well-characterized populations. The Core has also continued its focus on high-quality assays for animal-derived specimens and continues its development of targeted assays for metabolites in tissues. To provide Als with access to cutting edge technology in discovery metabolomics and micro-RNA analyses, in response both to requests of current Als and anticipated future requests, we have developed a new Discovery Metabolomics Subcore. The major changes in the Core are: ? Development of high-throughput targeted proteomic and metabolomic assays ? Establishment of a Discovery Metabolomics Subcore

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZDK1-GRB-2)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Washington
United States
Zip Code
Deem, Jennifer D; Muta, Kenjiro; Ogimoto, Kayoko et al. (2018) Leptin regulation of core body temperature involves mechanisms independent of the thyroid axis. Am J Physiol Endocrinol Metab 315:E552-E564
Freeman, Sara M; Ngo, Julie; Singh, Bhavdeep et al. (2018) Effects of Chronic Oxytocin Administration and Diet Composition on Oxytocin and Vasopressin 1a Receptor Binding in the Rat Brain. Neuroscience 392:241-251
Berkseth, Kathryn E; Rubinow, Katya B; Melhorn, Susan J et al. (2018) Hypothalamic Gliosis by MRI and Visceral Fat Mass Negatively Correlate with Plasma Testosterone Concentrations in Healthy Men. Obesity (Silver Spring) 26:1898-1904
Melhorn, Susan J; Askren, Mary K; Chung, Wendy K et al. (2018) FTO genotype impacts food intake and corticolimbic activation. Am J Clin Nutr 107:145-154
Ginos, Bigina N R; Navarro, Sandi L; Schwarz, Yvonne et al. (2018) Circulating bile acids in healthy adults respond differently to a dietary pattern characterized by whole grains, legumes and fruits and vegetables compared to a diet high in refined grains and added sugars: A randomized, controlled, crossover feeding stud Metabolism 83:197-204
Shao, Dan; Villet, Outi; Zhang, Zhen et al. (2018) Glucose promotes cell growth by suppressing branched-chain amino acid degradation. Nat Commun 9:2935
Rubinow, Katya B; Houston, Barbara; Wang, Shari et al. (2018) Androgen receptor deficiency in monocytes/macrophages does not alter adiposity or glucose homeostasis in male mice. Asian J Androl 20:276-283
den Hartigh, Laura J; Gao, Zhan; Goodspeed, Leela et al. (2018) Obese Mice Losing Weight Due to trans-10,cis-12 Conjugated Linoleic Acid Supplementation or Food Restriction Harbor Distinct Gut Microbiota. J Nutr 148:562-572
Selamet, Umut; Katz, Ronit; Ginsberg, Charles et al. (2018) Serum Calcitriol Concentrations and Kidney Function Decline, Heart Failure, and Mortality in Elderly Community-Living Adults: The Health, Aging, and Body Composition Study. Am J Kidney Dis 72:419-428
Wright, Davene R; Christakis, Dimitri A; Lozano, Paula et al. (2018) Healthy, Wealthy, and Wise? Exploring Parent Comparative Optimism About Future Child Outcomes. MDM Policy Pract 3:2381468318774776

Showing the most recent 10 out of 601 publications