CORE 4 - FLOW CYTOMETRY CORE ? ABSTRACT The Joslin Flow Cytometry Core's primary objective is to provide state-of-the-art flow cytometers and cell sorters, as well as expert operation and advice to its users, so that they can isolate, analyze, and study pure populations of cells from complex tissues, increase our understanding of diabetes and its complications, and ultimately develop treatments and cures for diabetes. Much of the research at Joslin focuses on gaining an understanding of type 1 and type 2 diabetes at the cellular level. This includes studies of the cellular development of pancreatic beta cells, studies of the development and inflammation of metabolically active muscle and adipose cells, and studies of the mediators and modifiers of autoimmunity toward beta cells. All of these research areas require analysis and isolation of well-defined, pure, populations of live cells. Fluorescence activated cell sorting is currently the best method for rapid isolation of very well defined and highly purified, live cells. The Joslin Flow Cytometry Core meets this need. Cell sorting technology is continuously evolving and improving, enabling new approaches to questions in diabetes research. Therefore, a second mission of the Flow Core is to continually update and modernize to offer the most cutting edge cell sorting technology to its users. In addition, because flow cytometry is traditionally thought of as an immunological tool, the Joslin Core's mission includes educational activities that enhance the use of flow cytometry in other research areas, bringing this technology to new users and stimulating new avenues of diabetes research. The specific goals of the Core are: 1. To offer Joslin researchers the use of reliable, well maintained, cutting edge, and cost-efficient cell sorting and analysis machines. 2. To provide training opportunities to Joslin researchers so that they can effectively use flow cytometers and cell sorters, and to continually educate users regarding new applications in flow cytometry that could benefit their research. 3. To update instrumentation in response to new and cutting-edge technology developments, including upgrades to existing instruments, addition of new instrumentation, and establishment of collaborative arrangements to help develop and evaluate new technology.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK036836-33
Application #
9701963
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2019-04-01
Budget End
2020-03-31
Support Year
33
Fiscal Year
2019
Total Cost
Indirect Cost
Name
Joslin Diabetes Center
Department
Type
DUNS #
071723084
City
Boston
State
MA
Country
United States
Zip Code
02215
Karatepe, Kutay; Zhu, Haiyan; Zhang, Xiaoyu et al. (2018) Proteinase 3 Limits the Number of Hematopoietic Stem and Progenitor Cells in Murine Bone Marrow. Stem Cell Reports 11:1092-1105
Sustarsic, Elahu G; Ma, Tao; Lynes, Matthew D et al. (2018) Cardiolipin Synthesis in Brown and Beige Fat Mitochondria Is Essential for Systemic Energy Homeostasis. Cell Metab 28:159-174.e11
Lessard, Sarah J; MacDonald, Tara L; Pathak, Prerana et al. (2018) JNK regulates muscle remodeling via myostatin/SMAD inhibition. Nat Commun 9:3030
Cardamone, Maria Dafne; Tanasa, Bogdan; Cederquist, Carly T et al. (2018) Mitochondrial Retrograde Signaling in Mammals Is Mediated by the Transcriptional Cofactor GPS2 via Direct Mitochondria-to-Nucleus Translocation. Mol Cell 69:757-772.e7
Solheim, Marie H; Winnay, Jonathon N; Batista, Thiago M et al. (2018) Mice Carrying a Dominant-Negative Human PI3K Mutation Are Protected From Obesity and Hepatic Steatosis but Not Diabetes. Diabetes 67:1297-1309
Stanford, Kristin I; Lynes, Matthew D; Takahashi, Hirokazu et al. (2018) 12,13-diHOME: An Exercise-Induced Lipokine that Increases Skeletal Muscle Fatty Acid Uptake. Cell Metab 27:1111-1120.e3
McGill, Dayna E; Volkening, Lisa K; Butler, Deborah A et al. (2018) Baseline Psychosocial Characteristics Predict Frequency of Continuous Glucose Monitoring in Youth with Type 1 Diabetes. Diabetes Technol Ther 20:434-439
Weir, Gordon C; Ehlers, Mario R; Harris, Kristina M et al. (2018) Alpha-1 antitrypsin treatment of new-onset type 1 diabetes: An open-label, phase I clinical trial (RETAIN) to assess safety and pharmacokinetics. Pediatr Diabetes 19:945-954
Qi, Weier; Li, Qian; Gordin, Daniel et al. (2018) Preservation of renal function in chronic diabetes by enhancing glomerular glucose metabolism. J Mol Med (Berl) 96:373-381
Adachi, Yusuke; De Sousa-Coelho, Ana Luisa; Harata, Ikue et al. (2018) l-Alanine activates hepatic AMP-activated protein kinase and modulates systemic glucose metabolism. Mol Metab 17:61-70

Showing the most recent 10 out of 1120 publications