The Molecular Biology Core laboratory is a newly-instituted core facility in the University of Virginia DERC. It was begun with the aim of facilitating the research activities of two groups within the DERC: 1) several new investigators whose interests are in the molecular biology of hormone action; and 2) established investigators who are beginning to apply recombinant DNA technology to their research programs. The purpose of this laboratory are: 1) to provide a centralized facility for the preparation of bacteriological media, agar plates and other supplies that are used routinely in recombinant DNA techniques; the purchase of supplies in bulk and the mass-production of media and plates will represent a significant savings, as we have demonstrated in the Tissue Culture Core Laboratory; 2) to provide centralized, costly equipment items that will be shared by several investigators, in order to minimize the cost of these items on individual grants; 3) to perform bacteriological operations for Center members, such as transformation, propagation, and storage of bacterial stocks, and the large-scale preparation of plasmids or phage; 4) to provide a source of expertise in recombinant DNA techniques for investigators who wish to apply these techniques to problems related to hormone action; 5) to interface with the Tissue Culture Core Laboratory, the Radioimmunoassay Core Laboratory, the Hybridoma Center, and the Immunogenetics Core Laboratory on projects related to the introduction and expression of cloned genes in mammalian cells. This Core Laboratory is crucial to ongoing projects in the laboratories of Drs. Lynch (Pharmacology), Garrison (Pharmacology), Pearson (Biochemistry), Hewlett (Medicine), Creutz (Pharmacology) and Williams (Anatomy and Cell Biology).

Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1990
Total Cost
Indirect Cost
Name
University of Virginia
Department
Type
DUNS #
001910777
City
Charlottesville
State
VA
Country
United States
Zip Code
22904
Earnhardt, Richard C; Veldhuis, Johannes D; Cornett, Greg et al. (2002) Pathophysiology of hyperinsulinemia following pancreas transplantation: altered pulsatile versus Basal insulin secretion and the role of specific transplant anatomy in dogs. Ann Surg 236:480-90; discussion 490-1
Lampl, M; Birch, L; Picciano, M F et al. (2001) Child factor in measurement dependability. Am J Hum Biol 13:548-57
Straume, M; Johnson, M L; Veldhuis, J D (1998) Statistically accurate estimation of hormone concentrations and associated uncertainties: methodology, validation, and applications. Clin Chem 44:116-23
Pak, Y; Hong, Y; Kim, S et al. (1998) In vivo chiro-inositol metabolism in the rat: a defect in chiro-inositol synthesis from myo-inositol and an increased incorporation of chiro-[3H]inositol into phospholipid in the Goto-Kakizaki (G.K) rat. Mol Cells 8:301-9
Friend, K; Iranmanesh, A; Login, I S et al. (1997) Pyridostigmine treatment selectively amplifies the mass of GH secreted per burst without altering GH burst frequency, half-life, basal GH secretion or the orderliness of GH release. Eur J Endocrinol 137:377-86
Reyes-Fuentes, A; Chavarria, M E; Aguilera, G et al. (1997) Deconvolution analysis of bioassayable LH secretion and half-life in men with idiopathic oligoasthenospermia. Int J Androl 20:118-25
Booth Jr, R A; Weltman, J Y; Yankov, V I et al. (1996) Mode of pulsatile follicle-stimulating hormone secretion in gonadal hormone-sufficient and -deficient women--a clinical research center study. J Clin Endocrinol Metab 81:3208-14
Veldhuis, J D (1996) Gender differences in secretory activity of the human somatotropic (growth hormone) axis. Eur J Endocrinol 134:287-95
Reyes-Fuentes, A; Chavarria, M E; Carrera, A et al. (1996) Alterations in pulsatile luteinizing hormone and follicle-stimulating hormone secretion in idiopathic oligoasthenospermic men: assessment by deconvolution analysis--a clinical research center study. J Clin Endocrinol Metab 81:524-9
Veldhuis, J D (1996) Neuroendocrine mechanisms mediating awakening of the human gonadotropic axis in puberty. Pediatr Nephrol 10:304-17

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