L-arginine is a nutritionally dispensable amino acid, which is involved in many physiologic processes including nitric oxide (NO) synthesis. The L-arginine/NO pathway is a part of the normal process of cardiovascular autoregulation and it is probably deficient in patients with essential hypertension. L-arginine supplement prevent the induction of hypertension in rat-sensitive models and produce hypotension in healthy humans when given intravenously. The benefit of L-arginine supplementation has not clearly been demonstrated in humans with essential hypertension. This study will explore the in vivo adaptation mechanisms involved in arginine metabolism and homeostasis and in whole body nitric oxide production (measured as NO excretion) in such patients. We will also correlate metabolic data with a complete and exhaustive clinical evaluation. Ten patients will receive a controlled diet with a normal L-arginine intake (56.1 mg/Kg/day) and then an arginine-supplemented (561 mg/Kg/day) isonitrogenous diet. Clinical outcome measures include daily blood pressure, as well as catecholamines, hANP, AVP, aldosterone, cGMP level and renin activity on day one and day seven of each diet period. At the end of each diet period, an 8h stable-isotopic labelled tracer study will be performed to evaluate the arginine, ornithine and urea fluxes as well s the NO production via measurement of nitrite/nitrate. This project will shed new light on the potential benefit of arginine supplement in diseases involving the L-arginine/NO pathway, such as essential hypertension. It will also define more completely the metabolic basis underlying body arginine homeostasis in human adults.

Project Start
1997-09-01
Project End
1998-08-31
Budget Start
1996-10-01
Budget End
1997-09-30
Support Year
4
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Massachusetts General Hospital
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02199
Gill, Corey M; Azevedo, Debora C; Oliveira, Adriana L et al. (2018) Sex differences in pericardial adipose tissue assessed by PET/CT and association with cardiometabolic risk. Acta Radiol 59:1203-1209
Gonzalez Herrera, Karina N; Zaganjor, Elma; Ishikawa, Yoshinori et al. (2018) Small-Molecule Screen Identifies De Novo Nucleotide Synthesis as a Vulnerability of Cells Lacking SIRT3. Cell Rep 22:1945-1955
Singhal, Vibha; Torre Flores, Landy P; Stanford, Fatima C et al. (2018) Differential associations between appendicular and axial marrow adipose tissue with bone microarchitecture in adolescents and young adults with obesity. Bone 116:203-206
Paynter, Nina P; Balasubramanian, Raji; Giulianini, Franco et al. (2018) Metabolic Predictors of Incident Coronary Heart Disease in Women. Circulation 137:841-853
Grinspoon, Steven (2018) NOVEL MECHANISMS AND ANTI-INFLAMMATORY STRATEGIES TO REDUCE CARDIOVASCULAR RISK IN HUMAN IMMUNODEFICIENCY VIRUS. Trans Am Clin Climatol Assoc 129:140-154
deFilippi, Chris; Lo, Janet; Christenson, Robert et al. (2018) Novel mediators of statin effects on plaque in HIV: a proteomics approach. AIDS 32:867-876
De Giuseppe, Rachele; Braschi, Valentina; Bosoni, David et al. (2018) Dietary underreporting in women affected by polycystic ovary syndrome: A pilot study. Nutr Diet :
Stanford, Fatima Cody; Toth, Alexander T; Shukla, Alpana P et al. (2018) Weight Loss Medications in Older Adults After Bariatric Surgery for Weight Regain or Inadequate Weight Loss: A Multicenter Study. Bariatr Surg Pract Patient Care 13:171-178
Syed, Sana; Iqbal, Najeeha T; Sadiq, Kamran et al. (2018) Serum anti-flagellin and anti-lipopolysaccharide immunoglobulins as predictors of linear growth faltering in Pakistani infants at risk for environmental enteric dysfunction. PLoS One 13:e0193768
Foldyna, Borek; Fourman, Lindsay T; Lu, Michael T et al. (2018) Sex Differences in Subclinical Coronary Atherosclerotic Plaque Among Individuals With HIV on Antiretroviral Therapy. J Acquir Immune Defic Syndr 78:421-428

Showing the most recent 10 out of 1081 publications