The long-term objective of this project is to delineate the role of various cytokines in acute liver injury, regeneration, and repair. The following questions will be addressed. (1) What is the role of transforming growth factor-Beta (TGF-Beta) and tumor necrosis factor (TNF) in acute hepatic damage, regeneration and repair? This work will involve the examination of two models of acute liver injury, CC1 4 and galactosamine-induced liver damage, and regeneration after partial hepatectomy. Changes in TGF-Beta and TNF gene expression will be analyzed by Northern blot hybridizations and nuclear run-on assays, and protein levels quantitated as well. (2) What are the effects of inhibiting the actions of TGF-Beta and TNF? TGF- Beta and TNF blocking antibodies will be administered to the animal models which will then be studied for changes in histology, gene expression, or survivability. (3) How do prostaglandin PGE 2 and dexamethasone affect liver injury and repair? PGE 2 and dexamethasone will be used in the in vivo models to determine these agents effects on acute liver injury and repair. By achieving these aims a better understanding of the mechanisms of acute liver injury and repair will exist. With this basic knowledge, proper therapeutic modalities for this entity will become apparent.
| Dulyaninova, Natalya G; Ruiz, Penelope D; Gamble, Matthew J et al. (2018) S100A4 regulates macrophage invasion by distinct myosin-dependent and myosin-independent mechanisms. Mol Biol Cell 29:632-642 |
| Kakabadze, Zurab; Kakabadze, Ann; Chakhunashvili, David et al. (2018) Decellularized human placenta supports hepatic tissue and allows rescue in acute liver failure. Hepatology 67:1956-1969 |
| Rao, Lu; Hülsemann, Maren; Gennerich, Arne (2018) Combining Structure-Function and Single-Molecule Studies on Cytoplasmic Dynein. Methods Mol Biol 1665:53-89 |
| Gong, Zhenwei; Tasset, Inmaculada; Diaz, Antonio et al. (2018) Humanin is an endogenous activator of chaperone-mediated autophagy. J Cell Biol 217:635-647 |
| Kale, Abhijit; Ji, Zhejun; Kiparaki, Marianthi et al. (2018) Ribosomal Protein S12e Has a Distinct Function in Cell Competition. Dev Cell 44:42-55.e4 |
| Caballero, Benjamin; Wang, Yipeng; Diaz, Antonio et al. (2018) Interplay of pathogenic forms of human tau with different autophagic pathways. Aging Cell 17: |
| Akiyama, Matthew J; Agyemang, Linda; Arnsten, Julia H et al. (2018) Rationale, design, and methodology of a trial evaluating three models of care for HCV treatment among injection drug users on opioid agonist therapy. BMC Infect Dis 18:74 |
| Willis, Ian M (2018) Maf1 phenotypes and cell physiology. Biochim Biophys Acta Gene Regul Mech 1861:330-337 |
| Wang, Tony Y; Portincasa, Piero; Liu, Min et al. (2018) Mouse models of gallstone disease. Curr Opin Gastroenterol 34:59-70 |
| Hodge, Dayle Q; Cui, Jihong; Gamble, Matthew J et al. (2018) Histone Variant MacroH2A1 Plays an Isoform-Specific Role in Suppressing Epithelial-Mesenchymal Transition. Sci Rep 8:841 |
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