Abstract

In the past year, the emphasis has advanced beyond the mechanics of doing eDNA microarrays toward quantitative real time PCR and a strong bioinformatics approach for analysis of microarray data. In addition, we were able to purchase an ABI Prism 7000 Sequence Detection System that can conduct real time PCR analysis. This instrument has found a broad base of use in the Liver Center and has greatly increased core usage. The quantitative real time PCR approach is used to confirm eDNA microarray data and as a transcription discovery tool. We have provided a full spectrum of support services for this instrument as outlined in the Progress Report. We have also expanded our bioinformatics services and have brought on-line second and third generation programs for processing microarray data. The laboratories of Drs. Charles and Leslie Rogler hired Dr. Raquel Norel to head up their bioinformatics efforts.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
2P30DK041296-16
Application #
6797585
Study Section
Special Emphasis Panel (ZDK1-GRB-8 (J1))
Project Start
2004-06-01
Project End
2009-05-31
Budget Start
2004-06-01
Budget End
2005-05-31
Support Year
16
Fiscal Year
2004
Total Cost
$151,419
Indirect Cost

  Institution

Name
Albert Einstein College of Medicine
Department
Type
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461

  Publications

Walters, Ryan O; Arias, Esperanza; Diaz, Antonio et al. (2018) Sarcosine Is Uniquely Modulated by Aging and Dietary Restriction in Rodents and Humans. Cell Rep 25:663-676.e6
Liu, Zhongbo; Apontes, Pasha; Fomenko, Ekaterina V et al. (2018) Mangiferin Accelerates Glycolysis and Enhances Mitochondrial Bioenergetics. Int J Mol Sci 19:
Tekirdag, Kumsal; Cuervo, Ana Maria (2018) Chaperone-mediated autophagy and endosomal microautophagy: Joint by a chaperone. J Biol Chem 293:5414-5424
Zhao, Rongbao; Najmi, Mitra; Aluri, Srinivas et al. (2018) Concentrative Transport of Antifolates Mediated by the Proton-Coupled Folate Transporter (SLC46A1); Augmentation by a HEPES Buffer. Mol Pharmacol 93:208-215
Amengual, Jaume; Guo, Liang; Strong, Alanna et al. (2018) Autophagy Is Required for Sortilin-Mediated Degradation of Apolipoprotein B100. Circ Res 122:568-582
Schneider, Michael; Kumar, Vivek; Nordstrøm, Lars Ulrik et al. (2018) Inhibition of Delta-induced Notch signaling using fucose analogs. Nat Chem Biol 14:65-71
Iqbal, Niloy Jafar; Lu, Zhonglei; Liu, Shun Mei et al. (2018) Cyclin-dependent kinase 4 is a preclinical target for diet-induced obesity. JCI Insight 3:
Galsgaard, Katrine D; Winther-Sørensen, Marie; Ørskov, Cathrine et al. (2018) Disruption of glucagon receptor signaling causes hyperaminoacidemia exposing a possible liver-alpha-cell axis. Am J Physiol Endocrinol Metab 314:E93-E103
Shen, Ling; Liu, Yin; Tso, Patrick et al. (2018) Silencing steroid receptor coactivator-1 in the nucleus of the solitary tract reduces estrogenic effects on feeding and apolipoprotein A-IV expression. J Biol Chem 293:2091-2101
Dulyaninova, Natalya G; Ruiz, Penelope D; Gamble, Matthew J et al. (2018) S100A4 regulates macrophage invasion by distinct myosin-dependent and myosin-independent mechanisms. Mol Biol Cell 29:632-642

Showing the most recent 10 out of 451 publications