The mission ofthe Genetic Engineering and Gene Therapy (GEGT) Core is to work cooperatively with Liver Research Center (LRC) Investigators to facilitate the development of genetic engineering reagents for in vitro and in vivo experiments.
The specific aims are to (1) generate customized oncoretroviral, lentiviral, adenoviral and non-viral vectors with integrating or episomal characteristics;(2) provide state-of-the-art reagents for site-specific gene insertion for cell marking, phenotypic correction/modification or down-regulating gene expression;(3) provide viral and non-viral vectors for reprogramming somatic cells to induced pluripotent stem (IPS) cells;(4) when appropriate, train investigators and their research personnel in generating gene transfer vectors in their own laboratories;(5) provide consultation for study design and, if appropriate, data analysis to the investigators. To fulfill these needs, the GEGT is established through a cooperative cost-sharing arrangement with the Gene Therapy Core of the Albert Einstein College of Medicine. The GEGT Core will function in close collaboration with other LRC Cores, as well as other institutional Shared Facilities, but the specialized services of the GEGT do not overlap with those offered by other Cores. The Core will benefit from the 25-yr experience of the Scientific Director, Jayanta Roy- Chowdhury, Professor of Medicine and Genetics in gene transfer in vivo and ex vivo for pathobiological and translational research, as well as the significant technological expertise of the Operations Director, Dr. Xia Wang. GEGT Core will add value to LRC investigators by enabling them to generate and use vectors and reagents that would be prohibitively expensive or technically difficult for individual laboratories to develop. It will also give LRC investigators priority for service and discounted chargeback rates. Resource sharing with other LRC Cores, as well as other institution-wide shared facilities will allow the Core to provide its services in an effective and cost-efficient manner. By supporting the multifaceted pathophysiological and translational research projects of the LRC members, as well as extramural investigators, the Core will be highly relevant to healthcare-related liver research.

Public Health Relevance

Microscopy and imaging enable liver investigators to detect and quantitate processes ranging from molecular interactions within cells to cell division in tissues. The potential for considerable insights provided by imaging approaches requires access to the latest technologies and the expertise to successfully employ them. The Imaging and Cell Structure Core provides Liver Research Investigators with state of the art instruments, analytical tools, reagents, and consultations to successfully incorporate imaging into their studies

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
2P30DK041296-26
Application #
8743570
Study Section
Special Emphasis Panel (ZDK1-GRB-8 (J2))
Project Start
2014-08-15
Project End
2019-06-30
Budget Start
2014-08-15
Budget End
2015-06-30
Support Year
26
Fiscal Year
2014
Total Cost
$37,902
Indirect Cost
$15,206
Name
Albert Einstein College of Medicine
Department
Type
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
Walters, Ryan O; Arias, Esperanza; Diaz, Antonio et al. (2018) Sarcosine Is Uniquely Modulated by Aging and Dietary Restriction in Rodents and Humans. Cell Rep 25:663-676.e6
Liu, Zhongbo; Apontes, Pasha; Fomenko, Ekaterina V et al. (2018) Mangiferin Accelerates Glycolysis and Enhances Mitochondrial Bioenergetics. Int J Mol Sci 19:
Tekirdag, Kumsal; Cuervo, Ana Maria (2018) Chaperone-mediated autophagy and endosomal microautophagy: Joint by a chaperone. J Biol Chem 293:5414-5424
Zhao, Rongbao; Najmi, Mitra; Aluri, Srinivas et al. (2018) Concentrative Transport of Antifolates Mediated by the Proton-Coupled Folate Transporter (SLC46A1); Augmentation by a HEPES Buffer. Mol Pharmacol 93:208-215
Amengual, Jaume; Guo, Liang; Strong, Alanna et al. (2018) Autophagy Is Required for Sortilin-Mediated Degradation of Apolipoprotein B100. Circ Res 122:568-582
Schneider, Michael; Kumar, Vivek; Nordstrøm, Lars Ulrik et al. (2018) Inhibition of Delta-induced Notch signaling using fucose analogs. Nat Chem Biol 14:65-71
Iqbal, Niloy Jafar; Lu, Zhonglei; Liu, Shun Mei et al. (2018) Cyclin-dependent kinase 4 is a preclinical target for diet-induced obesity. JCI Insight 3:
Galsgaard, Katrine D; Winther-Sørensen, Marie; Ørskov, Cathrine et al. (2018) Disruption of glucagon receptor signaling causes hyperaminoacidemia exposing a possible liver-alpha-cell axis. Am J Physiol Endocrinol Metab 314:E93-E103
Shen, Ling; Liu, Yin; Tso, Patrick et al. (2018) Silencing steroid receptor coactivator-1 in the nucleus of the solitary tract reduces estrogenic effects on feeding and apolipoprotein A-IV expression. J Biol Chem 293:2091-2101
Dulyaninova, Natalya G; Ruiz, Penelope D; Gamble, Matthew J et al. (2018) S100A4 regulates macrophage invasion by distinct myosin-dependent and myosin-independent mechanisms. Mol Biol Cell 29:632-642

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