The Tissue and Cell Analysis (TCA) Core of The University of Chicago DDRCC provides services vital to the study of intestinal disease, particularly immune-mediated, infectious, neoplastic and inflammatory bowel diseases, in human and animal tissues. In simplest terms, the core components can be broken into seven major areas: (1) anatomic pathology review and consultation;(2) processing of formalin-fixed paraffinembedded mouse tissues;(3) access to human tissues and tissue arrays from the paraffin-embedded tissue archives of the University of Chicago Department of Pathology;(4) high quality banking of snap-frozen tissues from surgical specimens;(5) training and use of laser capture micro-dissection microscopes and cryostats;(6) training and use of the ACIS II automated cellular imaging system;and (7) training and use of 2 photon, confocal, and widefield fluorescence microscopes as well as brightfield photomicroscopes. The TCA Core was created through a 2007 reorganization of the Molecular and Experimental Pathology (MEP) Core in an effort to better meet the needs of the DDRCC membership by integrating tissue processing, imaging, and analysis. This was stimulated by a survey of DDRCC membership, evaluation of core service use, and recognition that several key recommendations made by the reviewers in the 2005 review of this core (as part of the DDRCC renewal) were precisely correct. As was true of the previous MEP Core, the TCA Core has partnered with existing resources to generate significant cost-savings to both the core and the DDRCC membership. The Administrative Director of the Molecular Experimental Pathology Core, Dr. Jerrold Turner M.D., Ph.D., oversees the operations of all components and is directly involved in all human tissue-related operations, provides anatomic pathology review and consultation services, and supervises human and animal tissue immunostaining and tissue array production. The Co-Director of the TCA Core, Dr. Vytautas Bindokas, Ph.D., directs fluorescence microscopy facilities and provides direct assistance and education in image acquisition as well as off-line analysis.

Public Health Relevance

Analysis of human and animal tissues, as well as cultured cells, is required for advanced studies of intestinal disease. The Tissue and Cell Analysis Core provides state-of-the-art imaging resources, a rich library of archival human clinical specimens, essential standard services, and expert consultation to support DDRCC investigators.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK042086-24
Application #
8575334
Study Section
Special Emphasis Panel (ZDK1-GRB-8)
Project Start
Project End
Budget Start
2013-12-01
Budget End
2014-11-30
Support Year
24
Fiscal Year
2014
Total Cost
$101,300
Indirect Cost
$36,364
Name
University of Chicago
Department
Type
DUNS #
005421136
City
Chicago
State
IL
Country
United States
Zip Code
60637
Miyoshi, Jun; Leone, Vanessa; Nobutani, Kentaro et al. (2018) Minimizing confounders and increasing data quality in murine models for studies of the gut microbiome. PeerJ 6:e5166
Horton, Brendan L; Gajewski, Thomas F (2018) Back from the dead: TIL apoptosis in cancer immune evasion. Br J Cancer 118:309-311
Kroeger, Marie E; Delmont, Tom O; Eren, A M et al. (2018) New Biological Insights Into How Deforestation in Amazonia Affects Soil Microbial Communities Using Metagenomics and Metagenome-Assembled Genomes. Front Microbiol 9:1635
Berni Canani, Roberto; De Filippis, Francesca; Nocerino, Rita et al. (2018) Gut microbiota composition and butyrate production in children affected by non-IgE-mediated cow's milk allergy. Sci Rep 8:12500
Krugliak Cleveland, Noa; Rubin, David T; Hart, John et al. (2018) Patients With Ulcerative Colitis and Primary Sclerosing Cholangitis Frequently Have Subclinical Inflammation in the Proximal Colon. Clin Gastroenterol Hepatol 16:68-74
Pierre, J F; Hinterleitner, R; Bouziat, R et al. (2018) Data on changes to mucosal inflammation and the intestinal microbiota following dietary micronutrients in genetically susceptible hosts. Data Brief 20:387-393
Mayassi, Toufic; Jabri, Bana (2018) Human intraepithelial lymphocytes. Mucosal Immunol 11:1281-1289
Gilbert, Jack A; Jansson, Janet K; Knight, Rob (2018) Earth Microbiome Project and Global Systems Biology. mSystems 3:
Samanta, Priyanka; Wang, Yitang; Fuladi, Shadi et al. (2018) Molecular determination of claudin-15 organization and channel selectivity. J Gen Physiol 150:949-968
McDonald, Daniel; Hyde, Embriette; Debelius, Justine W et al. (2018) American Gut: an Open Platform for Citizen Science Microbiome Research. mSystems 3:

Showing the most recent 10 out of 697 publications