The Tissue Engineering and Cell Models Core (TECM) enables Universtiy of Chicago (UChicago) Digestive Diseases Research Core Center (DDRCC) for Interdisciplinary Study of Inflammatory Intestinal Disorders (C- IID) investigators to address key questions relating to causality and mechanisms by providing novel and vetted experimental models that can be customized to their needs. It was established to replace the Genomics and Molecular Engineering (GME) Core of the C-IID. The reorganization of the Core was a direct result of two sequential annual needs assessments indicating strong interest by the membership for more physiologically- relevant experimental models to study digestive health and diseases. The TECM has three major components: (1) A centralized repository and facility for established and primary gut-relevant cell lines, which was originally part of the host-microbe core, but moved to the TECM to centralize all cell and tissue experimental systems, (2) A tissue engineering component that includes customizing and developing intestinal organoid technologies for specific applications needed by C-IID users, and (3) an experimental systems component that includes in vivo models (rodent microsurgery), ex vivo models, and live functional assays. Inherent to these services, the TECM provides training and education, opportunities for cost-savings through bulk purchases (serum, disposables, etc), and technical expertise that saves investigators time and ensure high quality of services. The TECM is inextricably tied to the other C-IID cores. The Integrative Clinical and Biospecimen (ICB) and the Host-Microbe (HM) cores are essential for providing cells, tissues, and patient samples for establishing the experimental models. The Multiparametric Host Cell Analysis (MHC) core provides investigators with the means to analyze data derived from the model systems. Thus, the TECM Core has had great impact in enabling C-IID members to advance knowledge in the thematic areas fostered by the C-IID which focus on the study of IBD, host-microbe interactions, mucosal immunology and inflammation. In the past funding cycle, it was used by ~80% of the C-IID membership and was used in 149 peer-reviewed publications or 46% of the total (322) C-IID acknowledged publications during this period. The TECM has promoted interaction, collaboration, cost-sharing, and efficient resources utilization, and, at the same time, has enhanced the capabilities of our investigators.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
2P30DK042086-31
Application #
10049114
Study Section
Special Emphasis Panel (ZDK1)
Project Start
1996-12-01
Project End
2025-11-30
Budget Start
2020-12-01
Budget End
2021-11-30
Support Year
31
Fiscal Year
2021
Total Cost
Indirect Cost
Name
University of Chicago
Department
Type
DUNS #
005421136
City
Chicago
State
IL
Country
United States
Zip Code
60637
Wali, Ramesh K; Bianchi, Laura; Kupfer, Sonia et al. (2018) Prevention of colonic neoplasia with polyethylene glycol: A short term randomized placebo-controlled double-blinded trial. PLoS One 13:e0193544
Gaines, S; Shao, C; Hyman, N et al. (2018) Gut microbiome influences on anastomotic leak and recurrence rates following colorectal cancer surgery. Br J Surg 105:e131-e141
Pekow, Joel; Meckel, Katherine; Dougherty, Urszula et al. (2018) Increased mucosal expression of miR-215 precedes the development of neoplasia in patients with long-standing ulcerative colitis. Oncotarget 9:20709-20720
Bouziat, Romain; Biering, Scott B; Kouame, Elaine et al. (2018) Murine Norovirus Infection Induces TH1 Inflammatory Responses to Dietary Antigens. Cell Host Microbe 24:677-688.e5
Walker, Amy V; Gelb, Lev D; Barry, Grant E et al. (2018) Femtosecond laser desorption ionization mass spectrometry imaging and multivariate analysis of lipids in pancreatic tissue. Biointerphases 13:03B416
Wang, Yunwei; Lin, Fanfei; Zhu, Xiaorong et al. (2018) Distinct roles of intracellular heat shock protein 70 in maintaining gastrointestinal homeostasis. Am J Physiol Gastrointest Liver Physiol 314:G164-G178
Wang, Jingzhou; Bhatia, Arvin; Krugliak Cleveland, Noa et al. (2018) Rapid Onset of Inflammatory Bowel Disease after Receiving Secukinumab Infusion. ACG Case Rep J 5:e56
Banovich, Nicholas E; Li, Yang I; Raj, Anil et al. (2018) Impact of regulatory variation across human iPSCs and differentiated cells. Genome Res 28:122-131
Miyoshi, Jun; Leone, Vanessa; Nobutani, Kentaro et al. (2018) Minimizing confounders and increasing data quality in murine models for studies of the gut microbiome. PeerJ 6:e5166
Horton, Brendan L; Gajewski, Thomas F (2018) Back from the dead: TIL apoptosis in cancer immune evasion. Br J Cancer 118:309-311

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