One of the notable strengths of this Morphology Core is the broad range of morphological and Immunolocallzatlon techniques offered. A combination of techniques provides a complete spectrum of analysis progressing from the light microscopic level to the electron microscopic level for both morphological and immunocytochemlcal studies. Most recently, real time live cell imaging, total Internal reflection microscopy and laser cutting microdissection have been added to the core repertoire. These techniques allow Immunofluorescence and immunoperoxidase localization on whole cell monolayers, thin frozen sections of cells, tissues and Isolated membranes and vesicles. Techniques Include conventional, electron and confocal microscopy, localization on polarized cells, immunocytochemlcal localization at the ultrastructural level by immunoperoxidase and immunogold staining on Epon and Lowicryl embedded sections of tissue and cells (Including biopsy material), ultrathln frozen sections which allow the co-localization of pairs of proteins and TUNEL assay to study apoptosis. Through this core facility, CSIBD investigators have the opportunity to explore several different methods for observing the ultrastructural morphology of cells and tissues and for localizing proteins in these specimens. Over the previous funding period, investigators have used our Perkin-Elmer Live Cell Imager, a spinning disk confocal microscope to analyze cellular events as they occur In real time. By making these techniques available to investigators whose expertise lies in other areas, the Morphology Core serves to encourage a multi-disciplinary approach to the study of Intestinal disease. Studies supported by the Morphology Core during the current support period have resulted In 122 original publications in peer-reviewed journals listed at the end of the description of this core. Since the formation of the center. Dr. AtuI K. Bhan has served as Core Director, a role in which he will continue throughout the renewal period. Dr. Dennis Brown, Co-Director, an Internationally recognized expert in morphologic approaches to the study of epithelial cells, Is responsible for electron and confocal microscopy and related services of the core. The recent addition of Dr. Yukako Yagi who directs the Advanced Pathology Imaging Laboratory to the Morphology Core as an Associate Core Director, has added another dimension to the Core of providing sophisticated digital technology for the Center investigators. The core technicians are highly experienced microscopists and can frequently help Investigators without the Intervention of the Core Directors. With the expertise In operation and management of the Morphology Core over the past 18 years and the continuous molding of the Core In response to the evolving needs of the increasing base of CSIBD Investigators, evolutionary modifications in the services provided by the Core have been made and more are planned (See below). As a result of increased need for preparation of H&E stained paraffin-embedded sections of tissues for histology and pathology, as well as immunohistochemical studies on frozen tissue sections, greater resources will be directed to these services. Indeed, two new Leica cryostats were purchased three years ago to address this issue. This increased need resulted from the growing use of murine models of IBD established through gene deletion, mice made available through the Genetic Animal Model Core (e.g., the laboratories of Drs. Bhan, Atsushl Mizoguchi, Avruch, Terhorst, Snapper, Lacy-Hulbert, Nanthakumar, Xavier and others) and the development of a variety of transgenic mouse lines to explore IBD-related factors and processes, especially with the Implementation of a transgenic mouse service by the Molecular Biology Core during the current support period.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK043351-24
Application #
8583309
Study Section
Special Emphasis Panel (ZDK1-GRB-8)
Project Start
Project End
Budget Start
2014-01-01
Budget End
2014-12-31
Support Year
24
Fiscal Year
2014
Total Cost
$98,388
Indirect Cost
$44,037
Name
Massachusetts General Hospital
Department
Type
DUNS #
073130411
City
Boston
State
MA
Country
United States
Zip Code
02199
Ganna, Andrea; Satterstrom, F Kyle; Zekavat, Seyedeh M et al. (2018) Quantifying the Impact of Rare and Ultra-rare Coding Variation across the Phenotypic Spectrum. Am J Hum Genet 102:1204-1211
Vandoorne, Katrien; Rohde, David; Kim, Hye-Yeong et al. (2018) Imaging the Vascular Bone Marrow Niche During Inflammatory Stress. Circ Res 123:415-427
Cai, Tianrun; Lin, Tzu-Chieh; Bond, Allison et al. (2018) The Association Between Arthralgia and Vedolizumab Using Natural Language Processing. Inflamm Bowel Dis 24:2242-2246
Sayeed, Md Abu; Islam, Kamrul; Hossain, Motaher et al. (2018) Development of a new dipstick (Cholkit) for rapid detection of Vibrio cholerae O1 in acute watery diarrheal stools. PLoS Negl Trop Dis 12:e0006286
Maehara, Takashi; Mattoo, Hamid; Mahajan, Vinay S et al. (2018) The expansion in lymphoid organs of IL-4+ BATF+ T follicular helper cells is linked to IgG4 class switching in vivo. Life Sci Alliance 1:
Kearney, Sean M; Gibbons, Sean M; Poyet, Mathilde et al. (2018) Endospores and other lysis-resistant bacteria comprise a widely shared core community within the human microbiota. ISME J 12:2403-2416
Islam, Kamrul; Hossain, Motaher; Kelly, Meagan et al. (2018) Anti-O-specific polysaccharide (OSP) immune responses following vaccination with oral cholera vaccine CVD 103-HgR correlate with protection against cholera after infection with wild-type Vibrio cholerae O1 El Tor Inaba in North American volunteers. PLoS Negl Trop Dis 12:e0006376
Lidofsky, Anna; Holmes, Jacinta A; Feeney, Eoin R et al. (2018) Macrophage Activation Marker Soluble CD163 Is a Dynamic Marker of Liver Fibrogenesis in Human Immunodeficiency Virus/Hepatitis C Virus Coinfection. J Infect Dis 218:1394-1403
Kruger, Annie J; Fuchs, Bryan C; Masia, Ricard et al. (2018) Prolonged cenicriviroc therapy reduces hepatic fibrosis despite steatohepatitis in a diet-induced mouse model of nonalcoholic steatohepatitis. Hepatol Commun 2:529-545
Moran, Christopher J; Huang, Hailiang; Rivas, Manuel et al. (2018) Genetic variants in cellular transport do not affect mesalamine response in ulcerative colitis. PLoS One 13:e0192806

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