Abstract

(Taken directly from the application) The Vector Core proposes to be a resource for Centers participants. The objectives include consultation services, provision of existing vectors (retroviral , AAV and adenoviral), generation of new vectors, production of vectors for clinical trials, maintenance of GLP condition, training of investigators, improvements in existing technologies, and testing of new vector designs in animals.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK047754-09
Application #
6564287
Study Section
Project Start
2002-01-01
Project End
2002-12-31
Budget Start
Budget End
Support Year
9
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
University of Washington
Department
Type
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Murnane, R; Zhang, X B; Hukkanen, R R et al. (2011) Myelodysplasia in 2 pig-tailed macaques (Macaca nemestrina) associated with retroviral vector-mediated insertional mutagenesis and overexpression of HOXB4. Vet Pathol 48:999-1001
Metzger, Michael J; McConnell-Smith, Audrey; Stoddard, Barry L et al. (2011) Single-strand nicks induce homologous recombination with less toxicity than double-strand breaks using an AAV vector template. Nucleic Acids Res 39:926-35
Miller, A D; Metzger, M J (2011) APOBEC3-mediated hypermutation of retroviral vectors produced from some retrovirus packaging cell lines. Gene Ther 18:528-30
Halbert, C L; Metzger, M J; Lam, S-L et al. (2011) Capsid-expressing DNA in AAV vectors and its elimination by use of an oversize capsid gene for vector production. Gene Ther 18:411-7
Kiem, Hans-Peter; Ironside, Christina; Beard, Brian C et al. (2010) A retroviral vector common integration site between leupaxin and zinc finger protein 91 (ZFP91) observed in baboon hematopoietic repopulating cells. Exp Hematol 38:819-22, 822.e1-3
Trobridge, Grant D; Wu, Robert A; Hansen, Michael et al. (2010) Cocal-pseudotyped lentiviral vectors resist inactivation by human serum and efficiently transduce primate hematopoietic repopulating cells. Mol Ther 18:725-33
Becker, P S; Taylor, J A; Trobridge, G D et al. (2010) Preclinical correction of human Fanconi anemia complementation group A bone marrow cells using a safety-modified lentiviral vector. Gene Ther 17:1244-52
Doty, Raymond T; Sabo, Kathleen M; Chen, Jing et al. (2010) An all-feline retroviral packaging system for transduction of human cells. Hum Gene Ther 21:1019-27
Enssle, Joerg; Trobridge, Grant D; Keyser, Kirsten A et al. (2010) Stable marking and transgene expression without progression to monoclonality in canine long-term hematopoietic repopulating cells transduced with lentiviral vectors. Hum Gene Ther 21:397-403
Halbert, Christine L; Madtes, David K; Vaughan, Andrew E et al. (2010) Expression of human alpha1-antitrypsin in mice and dogs following AAV6 vector-mediated gene transfer to the lungs. Mol Ther 18:1165-72

Showing the most recent 10 out of 176 publications