Genetic modification of hematopoietic cells has many potential clinical applications, however, many investigators do not have the facilities or the technologies to perform and analyze transduction studies. Thus the major goal of the Hematopoietic Cell Transduction Core (HCTC) is to facilitate gene transfer studies to hematopoietic cells. Specifically the HCTC has 3 major objectives: 1) provision of a facility to perform transduction experiments for hematopoietic cells from dogs, baboons and humans; 2) assistance for investigators with the analysis of hematopoietic cell transduction experiments; and 3) improvement of current gene transfer methods and the development of improved technologies to analyze gene modified cells. The HCTC director has extensive experience with the transduction of hematopoietic cells in preclinical and in clinical settings. The HCTC director has more than 300 sq.ft for cell culture procedures and approximately 600 sq.ft for general molecular biology. The HCTC has also access to the shared space within the Transplantation Biology Program which consists of an additional 400 sq. ft of tissue culture space and more than 2,000 sq. ft. of fully equipped laboratory space. In addition, the HCTC has access to our cGMP Cell Processing Facility, which consists of approximately 4,500 square feet of dedicated QA/QC laboratory space for clinical cell manipulations and gene therapy studies. In summary, the HCTC will allow investigators to efficiently and effectively study their gene transfer strategies in hematopoietic cells and move their funded gene therapy related projects from preclinical studies to clinical gene therapy studies.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
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Special Emphasis Panel (ZDK1)
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University of Washington
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Murnane, R; Zhang, X B; Hukkanen, R R et al. (2011) Myelodysplasia in 2 pig-tailed macaques (Macaca nemestrina) associated with retroviral vector-mediated insertional mutagenesis and overexpression of HOXB4. Vet Pathol 48:999-1001
Metzger, Michael J; McConnell-Smith, Audrey; Stoddard, Barry L et al. (2011) Single-strand nicks induce homologous recombination with less toxicity than double-strand breaks using an AAV vector template. Nucleic Acids Res 39:926-35
Miller, A D; Metzger, M J (2011) APOBEC3-mediated hypermutation of retroviral vectors produced from some retrovirus packaging cell lines. Gene Ther 18:528-30
Halbert, C L; Metzger, M J; Lam, S-L et al. (2011) Capsid-expressing DNA in AAV vectors and its elimination by use of an oversize capsid gene for vector production. Gene Ther 18:411-7
Kiem, Hans-Peter; Ironside, Christina; Beard, Brian C et al. (2010) A retroviral vector common integration site between leupaxin and zinc finger protein 91 (ZFP91) observed in baboon hematopoietic repopulating cells. Exp Hematol 38:819-22, 822.e1-3
Trobridge, Grant D; Wu, Robert A; Hansen, Michael et al. (2010) Cocal-pseudotyped lentiviral vectors resist inactivation by human serum and efficiently transduce primate hematopoietic repopulating cells. Mol Ther 18:725-33
Becker, P S; Taylor, J A; Trobridge, G D et al. (2010) Preclinical correction of human Fanconi anemia complementation group A bone marrow cells using a safety-modified lentiviral vector. Gene Ther 17:1244-52
Doty, Raymond T; Sabo, Kathleen M; Chen, Jing et al. (2010) An all-feline retroviral packaging system for transduction of human cells. Hum Gene Ther 21:1019-27
Enssle, Joerg; Trobridge, Grant D; Keyser, Kirsten A et al. (2010) Stable marking and transgene expression without progression to monoclonality in canine long-term hematopoietic repopulating cells transduced with lentiviral vectors. Hum Gene Ther 21:397-403
Halbert, Christine L; Madtes, David K; Vaughan, Andrew E et al. (2010) Expression of human alpha1-antitrypsin in mice and dogs following AAV6 vector-mediated gene transfer to the lungs. Mol Ther 18:1165-72

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