Abstract

The zebrafish is a relatively new and extremely powerful genetic system for studying vertebrate development (Driever et al. 1996). As zebrafish are small, >70 can be maintained in a five gallon tank. Each mother produces >400 eggs weekly. Hence, large-scale genetics can be performed with ease. At Children's Hospital we have pioneered use of the zebrafish system for hematopoiesis. Two large scale screens for developmental mutants have produced >50 independent mutants affecting blood cell formation (Ransom et. al. 1996). These mutants have been collected at Children's Hospital. Presently, this CORE is a repository for zebrafish mutants of hematopoiesis, and supports their characterization and distribution.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK049216-07
Application #
6340875
Study Section
Project Start
2000-09-01
Project End
2001-08-31
Budget Start
Budget End
Support Year
7
Fiscal Year
2000
Total Cost
$271,667
Indirect Cost

  Institution

Name
Children's Hospital Boston
Department
Type
DUNS #
076593722
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Vo, Linda T; Kinney, Melissa A; Liu, Xin et al. (2018) Regulation of embryonic haematopoietic multipotency by EZH1. Nature 553:506-510
Mandelbaum, Joseph; Shestopalov, Ilya A; Henderson, Rachel E et al. (2018) Zebrafish blastomere screen identifies retinoic acid suppression of MYB in adenoid cystic carcinoma. J Exp Med 215:2673-2685
Yamauchi, Takuji; Masuda, Takeshi; Canver, Matthew C et al. (2018) Genome-wide CRISPR-Cas9 Screen Identifies Leukemia-Specific Dependence on a Pre-mRNA Metabolic Pathway Regulated by DCPS. Cancer Cell 33:386-400.e5
Blaser, Bradley W; Zon, Leonard I (2018) Making HSCs in vitro: don't forget the hemogenic endothelium. Blood 132:1372-1378
Cesana, Marcella; Guo, Michael H; Cacchiarelli, Davide et al. (2018) A CLK3-HMGA2 Alternative Splicing Axis Impacts Human Hematopoietic Stem Cell Molecular Identity throughout Development. Cell Stem Cell 22:575-588.e7
Lessard, Samuel; Francioli, Laurent; Alfoldi, Jessica et al. (2017) Human genetic variation alters CRISPR-Cas9 on- and off-targeting specificity at therapeutically implicated loci. Proc Natl Acad Sci U S A 114:E11257-E11266
Perlin, Julie R; Sporrij, Audrey; Zon, Leonard I (2017) Blood on the tracks: hematopoietic stem cell-endothelial cell interactions in homing and engraftment. J Mol Med (Berl) 95:809-819
Henninger, Jonathan; Santoso, Buyung; Hans, Stefan et al. (2017) Clonal fate mapping quantifies the number of haematopoietic stem cells that arise during development. Nat Cell Biol 19:17-27
Theodore, Lindsay N; Hagedorn, Elliott J; Cortes, Mauricio et al. (2017) Distinct Roles for Matrix Metalloproteinases 2 and 9 in Embryonic Hematopoietic Stem Cell Emergence, Migration, and Niche Colonization. Stem Cell Reports 8:1226-1241
Canver, Matthew C; Bauer, Daniel E; Orkin, Stuart H (2017) Functional interrogation of non-coding DNA through CRISPR genome editing. Methods 121-122:118-129

Showing the most recent 10 out of 98 publications