Abstract

This core provides a centralized laboratory charged with basic science research responsibilities and clinical services for the adult and pediatric hematology and stem cell transplantation programs. This core laboratory performs pre-clinical studies aimed at generating clinically relevant information essential for the development of clinical protocols and performs all of the processing procedures required for all stem cell components and for the preparation of autologous and allogeneic stem cell grafts used in translational research. In the area of pre-clinical studies, the stem cell laboratory examines the feasibility of proposed concepts and evaluates the effects of various manipulations on the quality and quantity of stem cell grafts. This centralized laboratory provides various members of the center access to hematopoietic stem cells from normal donors and patients for research purposes in a cost-effective environment. It facilitates the adoption and development of original laboratory techniques for use in stem cell transplantation and gene therapy. The core permits the administration of clinical trials in an environment providing quality control based manipulation of stem cells adhering to state and federal regulatory requirements and guidelines.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK049218-08
Application #
6496295
Study Section
Project Start
2001-09-01
Project End
2002-08-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
8
Fiscal Year
2001
Total Cost
$228,564
Indirect Cost

  Institution

Name
Indiana University-Purdue University at Indianapolis
Department
Type
DUNS #
005436803
City
Indianapolis
State
IN
Country
United States
Zip Code
46202

  Publications

Hoggatt, Jonathan; Singh, Pratibha; Tate, Tiffany A et al. (2018) Rapid Mobilization Reveals a Highly Engraftable Hematopoietic Stem Cell. Cell 172:191-204.e10
Xu, Linlin; Mohammad, Khalid S; Wu, Hao et al. (2016) Cell Adhesion Molecule CD166 Drives Malignant Progression and Osteolytic Disease in Multiple Myeloma. Cancer Res 76:6901-6910
Prasain, Nutan; Lee, Man Ryul; Vemula, Sasidhar et al. (2014) Differentiation of human pluripotent stem cells to cells similar to cord-blood endothelial colony-forming cells. Nat Biotechnol 32:1151-1157
Waning, David L; Li, Binghui; Jia, Nan et al. (2008) Cul4A is required for hematopoietic cell viability and its deficiency leads to apoptosis. Blood 112:320-9
Cai, Shanbao; Hartwell, Jennifer R; Cooper, Ryan J et al. (2006) In vivo effects of myeloablative alkylator therapy on survival and differentiation of MGMTP140K-transduced human G-CSF-mobilized peripheral blood cells. Mol Ther 13:1016-26
Li, Xiaxin; Le Beau, Michelle M; Ciccone, Samantha et al. (2005) Ex vivo culture of Fancc-/- stem/progenitor cells predisposes cells to undergo apoptosis, and surviving stem/progenitor cells display cytogenetic abnormalities and an increased risk of malignancy. Blood 105:3465-71
Srour, Edward F; Tong, Xia; Sung, Ki Woong et al. (2005) Modulation of in vitro proliferation kinetics and primitive hematopoietic potential of individual human CD34+CD38-/lo cells in G0. Blood 105:3109-16
Rinne, M L; He, Y; Pachkowski, B F et al. (2005) N-methylpurine DNA glycosylase overexpression increases alkylation sensitivity by rapidly removing non-toxic 7-methylguanine adducts. Nucleic Acids Res 33:2859-67
Tell, Gianluca; Damante, Giuseppe; Caldwell, David et al. (2005) The intracellular localization of APE1/Ref-1: more than a passive phenomenon? Antioxid Redox Signal 7:367-84
Bijangi-Vishehsaraei, Khadijeh; Saadatzadeh, M Reza; Werne, Adam et al. (2005) Enhanced TNF-alpha-induced apoptosis in Fanconi anemia type C-deficient cells is dependent on apoptosis signal-regulating kinase 1. Blood 106:4124-30

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