Cell Morphology and Pathology Core: The goals of the Cell Morphology and Pathology Core are to provide services and instruction to investigators of the Gene Therapy Center and to new investigators interested in developing gene transfer approaches to cystic fibrosis (CF) and other genetic diseases. We strive to provide expert assistance in the interpretation of disease pathology as investigators use gene transfer tools in new large and small animal models of human disease. To facilitate these goals the Core provides: 1) technical assistance for labor- intensive techniques such as the preparation of sections from tissue or cell cultures, 2) ready access to equipment, 3) economic benefits through centralization of equipment and facilities, and 4) consultation and instruction in specialized morphologic techniques and methods of image analysis. These goals are met through oversight by the Core Director, an established CF scientist with experience in a variety of microscopy techniques that are useful in gene therapy research.
The specific aims of the Cell Morphology and Pathology Core are: 1) To provide appropriate methods of tissue fixation, processing, cryopreservation, paraffin or plastic embedding, sectioning and routine staining for Center investigators. 2) For investigators who require detection of reporter proteins, to provide assistance with staining techniques, such as detection and cell-specific localization of (J-galactosidase activity. 3) To provide assistance with antibody labeling of proteins using histochemical and fluorescence techniques to investigators who require cell and tissue detection of specific proteins. 4) For investigators who require electron microscopy (TEM or SEM) or confocal microscopy, to provide tissue fixation, processing, and assistance in photo micrography, computerized analysis and interpretation of results. 5) For investigators who require in situ hybridization, to provide instruction and assistance in tissue preparation and detection of the probe. 6) To provide expert veterinary pathology support for the examination and interpretation of microscopic to gross specimens in animal models of disease and their response to gene-based therapies. 7) To provide assistance to investigators in the development and use of new and/or specialized morphological methods relevant to gene transfer.
|Martinez, Carlos H; Li, Sara X; Hirzel, Andrew J et al. (2018) Alveolar eosinophilia in current smokers with chronic obstructive pulmonary disease in the SPIROMICS cohort. J Allergy Clin Immunol 141:429-432|
|Ash, Samuel Y; Rahaghi, Farbod N; Come, Carolyn E et al. (2018) Pruning of the Pulmonary Vasculature in Asthma. The Severe Asthma Research Program (SARP) Cohort. Am J Respir Crit Care Med 198:39-50|
|Reznikov, Leah R; Meyerholz, David K; Kuan, Shin-Ping et al. (2018) Solitary Cholinergic Stimulation Induces Airway Hyperreactivity and Transcription of Distinct Pro-inflammatory Pathways. Lung 196:219-229|
|Meyerholz, David K; Sieren, Jessica C; Beck, Amanda P et al. (2018) Approaches to Evaluate Lung Inflammation in Translational Research. Vet Pathol 55:42-52|
|Schmidt, Megan E; Knudson, Cory J; Hartwig, Stacey M et al. (2018) Memory CD8 T cells mediate severe immunopathology following respiratory syncytial virus infection. PLoS Pathog 14:e1006810|
|Hua, Xiaoyang; Vijay, Rahul; Channappanavar, Rudragouda et al. (2018) Nasal priming by a murine coronavirus provides protective immunity against lethal heterologous virus pneumonia. JCI Insight 3:|
|Abou Alaiwa, Mahmoud H; Launspach, Jan L; Grogan, Brenda et al. (2018) Ivacaftor-induced sweat chloride reductions correlate with increases in airway surface liquid pH in cystic fibrosis. JCI Insight 3:|
|Lynch, Thomas J; Anderson, Preston J; Rotti, Pavana G et al. (2018) Submucosal Gland Myoepithelial Cells Are Reserve Stem Cells That Can Regenerate Mouse Tracheal Epithelium. Cell Stem Cell 22:653-667.e5|
|Hornick, Emma E; Banoth, Balaji; Miller, Ann M et al. (2018) Nlrp12 Mediates Adverse Neutrophil Recruitment during Influenza Virus Infection. J Immunol 200:1188-1197|
|Witry, Matthew; Comellas, Alejandro; Simmering, Jacob et al. (2018) The Association Between Technology Use and Health Status in a Chronic Obstructive Pulmonary Disease Cohort: Multi-Method Study. J Med Internet Res 20:e125|
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