Cell Morphology and Pathology Core: The goals of the Cell Morphology and Pathology Core are to provide services and instruction to investigators of the Gene Therapy Center and to new investigators interested in developing gene transfer approaches to cystic fibrosis (CF) and other genetic diseases. We strive to provide expert assistance in the interpretation of disease pathology as investigators use gene transfer tools in new large and small animal models of human disease. To facilitate these goals the Core provides: 1) technical assistance for labor- intensive techniques such as the preparation of sections from tissue or cell cultures, 2) ready access to equipment, 3) economic benefits through centralization of equipment and facilities, and 4) consultation and instruction in specialized morphologic techniques and methods of image analysis. These goals are met through oversight by the Core Director, an established CF scientist with experience in a variety of microscopy techniques that are useful in gene therapy research.
The specific aims of the Cell Morphology and Pathology Core are: 1) To provide appropriate methods of tissue fixation, processing, cryopreservation, paraffin or plastic embedding, sectioning and routine staining for Center investigators. 2) For investigators who require detection of reporter proteins, to provide assistance with staining techniques, such as detection and cell-specific localization of (J-galactosidase activity. 3) To provide assistance with antibody labeling of proteins using histochemical and fluorescence techniques to investigators who require cell and tissue detection of specific proteins. 4) For investigators who require electron microscopy (TEM or SEM) or confocal microscopy, to provide tissue fixation, processing, and assistance in photo micrography, computerized analysis and interpretation of results. 5) For investigators who require in situ hybridization, to provide instruction and assistance in tissue preparation and detection of the probe. 6) To provide expert veterinary pathology support for the examination and interpretation of microscopic to gross specimens in animal models of disease and their response to gene-based therapies. 7) To provide assistance to investigators in the development and use of new and/or specialized morphological methods relevant to gene transfer.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK054759-14
Application #
8564800
Study Section
Special Emphasis Panel (ZDK1-GRB-1 (J2))
Project Start
1998-09-30
Project End
2014-03-31
Budget Start
2012-04-01
Budget End
2013-03-31
Support Year
14
Fiscal Year
2012
Total Cost
$168,881
Indirect Cost
$57,651
Name
University of Iowa
Department
Type
DUNS #
062761671
City
Iowa City
State
IA
Country
United States
Zip Code
52242
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