The Metabolism Core is a state-of-the art facility for assessing human energy expenditure, body composition,and risk for chronic metabolic disease. It provides expertise, resources, and training in: Energy expenditure (free-living energy expenditure by doubly-labeled water and isotope-ratio massspectrometry); Body composition and fat distribution [(underwater weighing and BodPod for measurement of wholebody density; dual-energy X-ray absorptiometry (DXA) for measurement of regional and whole-bodybone, fat, and soft lean tissue; isotope dilution for assessment of total body water; multi-compartmentmodels of body composition; analysis of regional body composition (intra-abdominal and subcutaneousabdominal adipose tissue from computed tomography scans)]; Insulin sensitivity testing (frequently-sampled, intravenous, glucose tolerance test with minimalmodeling); oral glucose tolerance test with analysis of insulin and glucose; Analysis of glucose, lipids, and hormones (including obesity-related hormones, e.g., leptin, adiponectin;diabetes-related hormones, e.g., insulin, C-peptide, glucagon, GLP-1, GIP; a reproductive-endocrinepanel, e.g., estradiol, testosterone, other steroid hormones, gonadotropins; elements of the IGF system,e.g., IGF-1 and its binding proteins; cytokines and markers of inflammation, e.g., TNF-a and itsreceptors, IL-6, CRP); Analysis of isotopically-labeled glucose and amino acids by GC/MS for use in in vivo metabolic studies(e.g., endogenous glucose production, protein turnover).Since inception in 2000, Core A has supported 65 investigators and 102 research projects. Current core useincludes 24 investigators from 13 departments/divisions across DAB, and seven investigators from outsideof DAB. These investigators have a total of 47 funded projects that use Core services. Of these projects, 38(81%) are federally-funded. Two CNRU P/F studies presently use the Metabolism Core.New Methods Development includes, 1) the validation of a non-invasive test for insulin sensitivity in childrenusing a mixed-meal, and, 2) implementation of methodology for assessing insulin secretion and clearanceusing data resulting from a mixed-meal test.
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