Abstract

The Harvard Partners Center for Genetics and Genomics (HPCGG) Proteomics Facility offers high quality technical services for the analysis of proteins and peptides. The HPCGG currently has four Thermo ProteomeX LCQ workstations for multidimensional chromatography and ion trap mass spectrometry (LC-MS) for proteomic analysis, and bioinformatics tools for protein identification and biomarker validation. The facility can support the proteomic needs of the BADERC community through chromatographic and gel-based separation of intact proteins and proteolytic peptides, solution and in-gel digestion and LC-MS characterization of proteins, and relative quantification of proteins and peptides among samples. Zyomyx protein chip assays for mouse and human cytokines are also available. This Core is co-directed by David Sarracino, Ph.D., a biological chemist with academic and industrial experience in assay development, separations sciences and mass spectrometry, and Darryl Palmer-Toy, M.D., Ph.D., a board certified clinical pathologist with extensive experience in mass spectrometry and biomarker discovery.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK057521-09
Application #
7619460
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2008-04-01
Budget End
2009-03-31
Support Year
9
Fiscal Year
2008
Total Cost
$75,148
Indirect Cost

  Institution

Name
Massachusetts General Hospital
Department
Type
DUNS #
073130411
City
Boston
State
MA
Country
United States
Zip Code
02199

  Publications

McKeown, Nicola M; Dashti, Hassan S; Ma, Jiantao et al. (2018) Sugar-sweetened beverage intake associations with fasting glucose and insulin concentrations are not modified by selected genetic variants in a ChREBP-FGF21 pathway: a meta-analysis. Diabetologia 61:317-330
Vandoorne, Katrien; Rohde, David; Kim, Hye-Yeong et al. (2018) Imaging the Vascular Bone Marrow Niche During Inflammatory Stress. Circ Res 123:415-427
Ross, Rachel A; Leon, Silvia; Madara, Joseph C et al. (2018) PACAP neurons in the ventral premammillary nucleus regulate reproductive function in the female mouse. Elife 7:
Berkowitz, Seth A; Karter, Andrew J; Corbie-Smith, Giselle et al. (2018) Food Insecurity, Food ""Deserts,"" and Glycemic Control in Patients With Diabetes: A Longitudinal Analysis. Diabetes Care 41:1188-1195
Syed, Ismail; Lee, Jennifer; Moraes-Vieira, Pedro M et al. (2018) Palmitic Acid Hydroxystearic Acids Activate GPR40, Which Is Involved in Their Beneficial Effects on Glucose Homeostasis. Cell Metab 27:419-427.e4
Vujic, Ana; Lerchenmüller, Carolin; Wu, Ting-Di et al. (2018) Exercise induces new cardiomyocyte generation in the adult mammalian heart. Nat Commun 9:1659
Fulzele, Keertik; Dedic, Christopher; Lai, Forest et al. (2018) Loss of Gs? in osteocytes leads to osteopenia due to sclerostin induced suppression of osteoblast activity. Bone 117:138-148
Battistone, Maria A; Nair, Anil V; Barton, Claire R et al. (2018) Extracellular Adenosine Stimulates Vacuolar ATPase-Dependent Proton Secretion in Medullary Intercalated Cells. J Am Soc Nephrol 29:545-556
Cheung, Pui W; Terlouw, Abby; Janssen, Sam Antoon et al. (2018) Inhibition of non-receptor tyrosine kinase Src induces phosphoserine 256-independent aquaporin-2 membrane accumulation. J Physiol :
Karim, Lamya; Moulton, Julia; Van Vliet, Miranda et al. (2018) Bone microarchitecture, biomechanical properties, and advanced glycation end-products in the proximal femur of adults with type 2 diabetes. Bone 114:32-39

Showing the most recent 10 out of 389 publications