Rodent models of diabetes and its complications have and will continue to provide fundamental insights into the molecular basis of human metabolic disease. The investigators of the Columbia DRC employ many murine models to study diabetes and its complications. Characterizing the phenotype of these animals in an efficient, effective and timely fashion is critical for the productive study of diabetes at Columbia. The Mouse Metabolic Phenotype & Function Core (MMFP) has been in great demand during the current funding cycle and has evolved significantly since the last submission to meet the changing needs of DRC investigators. The MMFP has proven broadly successful in achieving its mission of assisting individual investigators in characterizing metabolic phenotypes of mice, fulfilling more than 18,000 service requests for 80 scientists in the laboratories of 22 DRC members, supported by 58 grants. The Core has been instrumental in obtaining 14 new grants, and in 72 publications (44 as primary Core). The MMFP currently provides services that facilitate the efficient characterization of mouse models of diabetes and its complications: NMR Body Composition Analysis, Whole Body Metabolic Assessment (chamber calorimetry with motion detection), Metabolic Clamps, Gastric Infusion/Feeding and Thermogenic Phenotyping. Importantly, Core personnel also provides guidance to investigators regarding selection of techniques and experimental design. The MMFP services complement those provided by other DRC Cores, so that investigators who take advantage of DRC resources can fully characterize the histologic, immunologic and metabolic function and phenotypes of mice. The Core has been built through the strong support of the Naomi Berrie Diabetes Center and Columbia University. To continue effectively serving the needs of the Columbia diabetes research community, the MMFP has evolved by adding and expanding services. In response to investigator surveys and as proposed in our last competing renewal, we developed three phenotyping services during the current funding period: Metabolic Clamps, Gastric Infusion Program and Thermogenic Phenotyping (controlled ambient temperature; cell and tissue calorimetry). These services are now well established. Projections indicate that demand for these Core services will remain high.

Public Health Relevance

The ability to perform advanced metabolic phenotyping of rodent models of diabetes, obesity, and insulin resistance, is essential to the operation of a modern Diabetes Research Center. The Mouse Metabolic Phenotype and Function Core has fulfilled this mission by offering high-quality characterization of mouse metabolism. With the implementation of new services this Core is poised to continue to provide critical support to DRC Activities.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK063608-19
Application #
10104484
Study Section
Special Emphasis Panel (ZDK1)
Project Start
2002-09-01
Project End
2023-01-31
Budget Start
2021-02-01
Budget End
2022-01-31
Support Year
19
Fiscal Year
2021
Total Cost
Indirect Cost
Name
Columbia University (N.Y.)
Department
Type
DUNS #
621889815
City
New York
State
NY
Country
United States
Zip Code
10032
Ghorpade, Devram S; Ozcan, Lale; Zheng, Ze et al. (2018) Hepatocyte-secreted DPP4 in obesity promotes adipose inflammation and insulin resistance. Nature 555:673-677
Connors, Thomas J; Baird, J Scott; Yopes, Margot C et al. (2018) Developmental Regulation of Effector and Resident Memory T Cell Generation during Pediatric Viral Respiratory Tract Infection. J Immunol 201:432-439
Savage, Thomas M; Shonts, Brittany A; Obradovic, Aleksandar et al. (2018) Early expansion of donor-specific Tregs in tolerant kidney transplant recipients. JCI Insight 3:
Molusky, Matthew M; Hsieh, Joanne; Lee, Samuel X et al. (2018) Metformin and AMP Kinase Activation Increase Expression of the Sterol Transporters ABCG5/8 (ATP-Binding Cassette Transporter G5/G8) With Potential Antiatherogenic Consequences. Arterioscler Thromb Vasc Biol 38:1493-1503
Carpenter, D J; Granot, T; Matsuoka, N et al. (2018) Human immunology studies using organ donors: Impact of clinical variations on immune parameters in tissues and circulation. Am J Transplant 18:74-88
Langlet, Fanny; Tarbier, Marcel; Haeusler, Rebecca A et al. (2018) microRNA-205-5p is a modulator of insulin sensitivity that inhibits FOXO function. Mol Metab 17:49-60
Proto, Jonathan D; Doran, Amanda C; Gusarova, Galina et al. (2018) Regulatory T Cells Promote Macrophage Efferocytosis during Inflammation Resolution. Immunity 49:666-677.e6
Carli, Jayne F Martin; LeDuc, Charles A; Zhang, Yiying et al. (2018) The role of Rpgrip1l, a component of the primary cilium, in adipocyte development and function. FASEB J 32:3946-3956
Postigo-Fernandez, Jorge; Creusot, RĂ©mi J (2018) A multi-epitope DNA vaccine enables a broad engagement of diabetogenic T cells for tolerance in Type 1 diabetes. J Autoimmun :
Proto, Jonathan D; Doran, Amanda C; Subramanian, Manikandan et al. (2018) Hypercholesterolemia induces T cell expansion in humanized immune mice. J Clin Invest 128:2370-2375

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