The goal of the Bio-Analytical Redox Biology (BARB) core is to provide state-of-the-art services in mitochondrial and cellular metabolism and oxidative stress assessment. Since its intiation in 2008, the BARB Core has provided services at a rate that has increased on average by 215% per year. New services will include the ex vivo measurement of cellular/tissue bioenergetics, mitochondrial metabolomics (citric acid cycle intermediates), and introduction of new measures of oxidative stress that will utilize 2-hydroxyethidium and related molecules employing mass spectrometry. These services will expand upon the current services and provide novel measures for research in diabetes and cardiometabolic disease.
The specific aims are: 1) Provide services to DRC Members and pilot &feasibility grant awardees in: (A) Mitochondrial metabolism: isolation, bioenergetics, membrane potential, ATP generation, oxidant production (amplex red), mt DNA damage;(B) Oxidative stress assessment (oxidized lipids, 3NT, aconitase inactivation, lipid peroxidation, LPO products, isoprostanes, GSH/GSSG, dihydroethidine and related compounds inactivation, S0D2 activity and GSH/GSSG);and (C) Cellular metabolism analyses - cell and tissue (permeabilized tissue - in situ/ex vivo) bioenergetics, citric acid cycle intermediate metabolomics and enzyme peptidomics. 2) To reduce cost and investigator time commitment by accessing the centralized resources of the core. 3) To provide investigators opportunities to learn and develop new research methodologies through recurring workshops and consultations that utilize BARB core services. 4) Development of new methodologies that extend research capabilities in redox and free radical biology.
These aims are based on the expressed needs of funded DRC investigators. The BARB core is unique within UAB and at NIDDK diabetes centers. The Core plans to continue the development of technologies to keep our investigators appraised of current developments in bioenergetics, metabolism, and oxidant stress quantification, via a continued outreach program consisting of tutorials and seminars.
The BARB Core is at the forefront of methods assessing mitochondrial metabolism, energetics, and oxidative stress, which are processes central to the pathogenesis of diabetes and cardiometabolic disease. The constellation of core services reflects an area of excellence in the DRC investigator base, and is unique among NIDDK diabetes centers and nation-wide. The Core will continue its outstanding and increasing record of service and facilitate advances pertaining to metabolic and vascular patholoav in diabetes.
|Ovalle, Fernando; Grimes, Tiffany; Xu, Guanlan et al. (2018) Verapamil and beta cell function in adults with recent-onset type 1 diabetes. Nat Med 24:1108-1112|
|Schneider, C R; Biggio, J R; Chandler-Laney, P C (2018) Association of early pregnancy body mass index with post-partum weight change among African-American women. Clin Obes 8:170-175|
|Kim, Teayoun; Nason, Shelly; Holleman, Cassie et al. (2018) Glucagon Receptor Signaling Regulates Energy Metabolism via Hepatic Farnesoid X Receptor and Fibroblast Growth Factor 21. Diabetes 67:1773-1782|
|Mohler 3rd, Emile R; Ellenberg, Susan S; Lewis, Cora E et al. (2018) The Effect of Testosterone on Cardiovascular Biomarkers in the Testosterone Trials. J Clin Endocrinol Metab 103:681-688|
|Loomis, Stephanie J; Li, Man; Maruthur, Nisa M et al. (2018) Genome-Wide Association Study of Serum Fructosamine and Glycated Albumin in Adults Without Diagnosed Diabetes: Results From the Atherosclerosis Risk in Communities Study. Diabetes 67:1684-1696|
|Wingo, Brooks C; Barry, Valene Garr; Ellis, Amy C et al. (2018) Comparison of segmental body composition estimated by bioelectrical impedance analysis and dual-energy X-ray absorptiometry. Clin Nutr ESPEN 28:141-147|
|Frugé, Andrew D; Cases, Mallory G; Howell, Carrie R et al. (2018) Fingernail and toenail clippings as a non-invasive measure of chronic cortisol levels in adult cancer survivors. Cancer Causes Control 29:185-191|
|Kim, Teayoun; Holleman, Cassie L; Nason, Shelly et al. (2018) Hepatic Glucagon Receptor Signaling Enhances Insulin-Stimulated Glucose Disposal in Rodents. Diabetes 67:2157-2166|
|Kreisler, A D; Mattock, M; Zorrilla, E P (2018) The duration of intermittent access to preferred sucrose-rich food affects binge-like intake, fat accumulation, and fasting glucose in male rats. Appetite 130:59-69|
|Sweatt, S Katherine; Gower, Barbara A; Chieh, Angela Y et al. (2018) Sleep quality is differentially related to adiposity in adults. Psychoneuroendocrinology 98:46-51|
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