The objective of the C-SiG Optical Microscopy Core, is to be a state-of-the-art, user-friendly service that connects investigators with the many optical technologies and applications at a reasonable cost. Under the direction of Dr. Mark McNiven, a well-established cell biologist, the Core integrates existing resources from the Mayo Microscopy and Cell Analysis Core and from individual investigators in the Division of Gastroenterology and Hepatology (GIH) as well as providing additional new technologies not previously available.
The Specific Aims of this core are three-fold. First, to provide reliable, accessible, state-of-the-art microscopic technology to all C-SiG members that will facilitate their study of Gl cellular signaling cascades. Second, to educate and train C-SiG members;in the use of both basic and sophisticated cellular imaging methods. Emphasis is placed on providing technical instruction as well as educating faculty on how such approaches can expand the scope and breadth of their scientific programs. Third, to develop and apply state-of-the-art optical imaging technologies, including fluorescent probes and biosensors, to study Gl tissues and/or cells. The most popular Core service is access to the well-maintained C-SiG Confocal Microscopes. The Core also provides instruction, technical advice, data interpretation, and development of novel, innovative optical approaches to the study of signaling pathways in Gl cells and tissues. These services cover a wide range of topics including: real-time computer/video imaging of live cells;confocal microscopy coupled with computer-based 3-D image reconstruction;Fluorescence Resonance Energy Transfer (FRET) applications to measure dynamic protein-protein interactions;Fluorescence Recovery After Photobleaching (FRAP) that allows the quantitation of protein recruitment/turnover;Fluorescence Loss in Photobleaching (FLIP);microinjection of living cells;expression and use of fluorescence-based bioprobes that facilitates the study and localization of specific signaling molecules including both proteins and lipids;the development and application of specific photo-activatable caged-compounds that allow a precise temporal and spatial activation of desired signaling molecules in live cells;Total internal reflection (TIRF) microscopy; multiphoton microscopy, and super-resolution microscopy. Core services have been used by 58% of CSiG members and supported 106 publications.

Public Health Relevance

Gastrointestinal diseases and their complications have a significant effect on public health and health care utilization costs. The C-SiG Optical Microscopy Core supports scientific advancements of C-SiG members that are critically important for furthering understanding of the mechanisms that underlie digestive diseases, which can lead to practical applications for the diagnosis, prevention, monitoring and treatment of human disease.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
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Special Emphasis Panel (ZDK1)
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Mayo Clinic, Rochester
United States
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Mansini, Adrian P; Lorenzo Pisarello, Maria J; Thelen, Kristen M et al. (2018) MicroRNA (miR)-433 and miR-22 dysregulations induce histone-deacetylase-6 overexpression and ciliary loss in cholangiocarcinoma. Hepatology 68:561-573
Moncsek, Anja; Al-Suraih, Mohammed S; Trussoni, Christy E et al. (2018) Targeting senescent cholangiocytes and activated fibroblasts with B-cell lymphoma-extra large inhibitors ameliorates fibrosis in multidrug resistance 2 gene knockout (Mdr2-/- ) mice. Hepatology 67:247-259
Allen, Alina M; Shah, Vijay H; Therneau, Terry M et al. (2018) The role of 3D-MRE in the diagnosis of NASH in obese patients undergoing bariatric surgery. Hepatology :
Dhanasekaran, Renumathy; Nault, Jean-Charles; Roberts, Lewis R et al. (2018) Genomic Medicine and Implications for Hepatocellular Carcinoma Prevention and Therapy. Gastroenterology :
Sugihara, Takaaki; Werneburg, Nathan W; Hernandez, Matthew C et al. (2018) YAP Tyrosine Phosphorylation and Nuclear Localization in Cholangiocarcinoma Cells Are Regulated by LCK and Independent of LATS Activity. Mol Cancer Res 16:1556-1567
Anderson, Bradley W; Suh, Yun-Suhk; Choi, Boram et al. (2018) Detection of Gastric Cancer with Novel Methylated DNA Markers: Discovery, Tissue Validation, and Pilot Testing in Plasma. Clin Cancer Res 24:5724-5734
Rizvi, Sumera; Fischbach, Samantha R; Bronk, Steven F et al. (2018) YAP-associated chromosomal instability and cholangiocarcinoma in mice. Oncotarget 9:5892-5905
Mouchli, Mohamad A; Ouk, Lidia; Scheitel, Marianne R et al. (2018) Colonoscopy surveillance for high risk polyps does not always prevent colorectal cancer. World J Gastroenterol 24:905-916
Rizvi, Sumera; Khan, Shahid A; Hallemeier, Christopher L et al. (2018) Cholangiocarcinoma - evolving concepts and therapeutic strategies. Nat Rev Clin Oncol 15:95-111
Hale, Vanessa L; Jeraldo, Patricio; Chen, Jun et al. (2018) Distinct microbes, metabolites, and ecologies define the microbiome in deficient and proficient mismatch repair colorectal cancers. Genome Med 10:78

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