The overall objective of the Histology/Imaging Core of the Cleveland DDRCC is to provide high-quality morphologic analyses of the gastrointestinal and liver systems in experimental animal models and human Gl and liver-related pathologies. In addition, the major goals of the Histology/Imaging Core is to support new and established investigators at Case Western Reserve University and the Cleveland Clinic Foundation who do not have a direct focus on digestive disease-related research to enter this exciting field of investigation and to encourage collaborations among DDRCC members working in the areas of digestive Inflammation and Metabolism. The Histology/Imaging Core is committed to providing basic and fundamental histological and immunohistochemical methodologies essential to Gl and liver-related research, while at the same time implementing state-of-the-art technologies, such as small animal imaging.
The specific aims proposed for the DDRCC Histology/Imaging Core are: 1) Provide basic, high-quality histologic and immunohistochemicai services in a cost-effect and timely fashion; 2) Provide initial service, consultation and subsequent training for special immunohistochemical staining in Gl and liver tissues; 3) Provide consistent and validated histological scoring of gastrointestinal and liver inflammation for preclinical and clinical studies; 4) Provide publication-ready photo documentation of acquired histological, immunohistochmical, and/or immunoflourescence-stained images; 5) Provide access to an animal tissue repository of histological blocks and slides of various models of Gl and liver inflammatory and metabolic disease; 6) Provide access to cutting-edge technologies for in vivo small animal imaging through a Small Animal Imaging SubCore to precisely address the extent, severity, and occurrence of lesions in animal models of gastrointestinal and liver inflammation and metabolism; and, 7) Provide consultation and training in all aspects of Gl-related morphology and imaging techniques. Through these aims, the Core will facilitate the production of high quality research in digestive diseases in a cost-effective and time-efficient manner, and enhance the rate, depth, and breadth of research in digestive Inflammation and Metabolism throughout CWRU and CCF.

Public Health Relevance

This Core aims to provide high-quality, cost-effective and time-efficient histological and imaging analysis of human and animal tissues of digestive inflammatory and metabolic diseases, such as IBD, hepatitis, metabolic syndromes, and obesity. These conditions affect millions of individuals in the US, and facilitating their study will increase the chance of uncovering key pathogenic mechansims and developing new therapeutic anoroaches

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK097948-05
Application #
9626399
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2019-02-01
Budget End
2020-01-31
Support Year
5
Fiscal Year
2019
Total Cost
Indirect Cost
Name
Case Western Reserve University
Department
Type
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106
Dziedzic, Slawomir A; Su, Zhenyi; Jean Barrett, Vica et al. (2018) ABIN-1 regulates RIPK1 activation by linking Met1 ubiquitylation with Lys63 deubiquitylation in TNF-RSC. Nat Cell Biol 20:58-68
Lopetuso, Loris R; De Salvo, Carlo; Pastorelli, Luca et al. (2018) IL-33 promotes recovery from acute colitis by inducing miR-320 to stimulate epithelial restitution and repair. Proc Natl Acad Sci U S A 115:E9362-E9370
Gu, Yuning; Wang, Charlie Y; Anderson, Christian E et al. (2018) Fast magnetic resonance fingerprinting for dynamic contrast-enhanced studies in mice. Magn Reson Med 80:2681-2690
Rodriguez-Palacios, Alexander; Harding, Andrew; Menghini, Paola et al. (2018) The Artificial Sweetener Splenda Promotes Gut Proteobacteria, Dysbiosis, and Myeloperoxidase Reactivity in Crohn's Disease-Like Ileitis. Inflamm Bowel Dis 24:1005-1020
Mehta, Kathan; Jaiswal, Palashkumar; Briggs, Farren et al. (2018) In-patient outcomes of Hematopoietic Stem Cell Transplantation in Patients with Immune Mediated Inflammatory Diseases: A Nationwide Study. Sci Rep 8:6825
Liu, Zhonghua; Wang, Chuanping; Rathkey, Joseph K et al. (2018) Structures of the Gasdermin D C-Terminal Domains Reveal Mechanisms of Autoinhibition. Structure 26:778-784.e3
Cooper, Gregory S; Markowitz, Sanford D; Chen, Zhengyi et al. (2018) Evaluation of Patients with an Apparent False Positive Stool DNA Test: The Role of Repeat Stool DNA Testing. Dig Dis Sci 63:1449-1453
Anderson, Christian E; Wang, Charlie Y; Gu, Yuning et al. (2018) Regularly incremented phase encoding - MR fingerprinting (RIPE-MRF) for enhanced motion artifact suppression in preclinical cartesian MR fingerprinting. Magn Reson Med 79:2176-2182
Perez, Jessica M; Chen, Yinghua; Xiao, Tsan S et al. (2018) Phosphorylation of the E3 ubiquitin protein ligase ITCH diminishes binding to its cognate E2 ubiquitin ligase. J Biol Chem 293:1100-1105
Petersen, Christine P; Meyer, Anne R; De Salvo, Carlo et al. (2018) A signalling cascade of IL-33 to IL-13 regulates metaplasia in the mouse stomach. Gut 67:805-817

Showing the most recent 10 out of 86 publications