Abstract

The overall objective of the Animal Models and Pathology Facilities Core is to provide Center members with state-of-the-art pathology support, transgenic resources and a centrally-managed AAALAC-approved animal holding and surgical facility. The Core is staffed with experienced personnel and is equipped with essential equipment to generate genetically engineered mice (GEM), rederive imported mice by embryo transfer rederivation, provide colony management, and prepare and interpret tissue samples by histological and image analysis.
The specific aims are as follows: (1) To generate transgenic animals by pronuclear microinjection of DNA constructs or embryonic stem cell manipulation and provide colony management of GEM for use by Center members; (2) To rederive transgenic and other specialized mouse strains by embryo transfer to assure specific pathogen free status of all mice used in this program; (3) To provide histology, pathology, molecular characterization and imaging expertise for extensive phenotyping and assessment of pathological damage of GEM and other animal models as needed.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Center Core Grants (P30)
Project #
5P30ES002109-30
Application #
7798630
Study Section
Environmental Health Sciences Review Committee (EHS)
Project Start
Project End
Budget Start
2009-04-01
Budget End
2010-03-31
Support Year
30
Fiscal Year
2009
Total Cost
$316,291
Indirect Cost

  Institution

Name
Massachusetts Institute of Technology
Department
Type
DUNS #
001425594
City
Cambridge
State
MA
Country
United States
Zip Code
02139

  Publications

Viswanathan, Srinivas R; Nogueira, Marina F; Buss, Colin G et al. (2018) Genome-scale analysis identifies paralog lethality as a vulnerability of chromosome 1p loss in cancer. Nat Genet 50:937-943
Co, Julia Y; Cárcamo-Oyarce, Gerardo; Billings, Nicole et al. (2018) Mucins trigger dispersal of Pseudomonas aeruginosa biofilms. NPJ Biofilms Microbiomes 4:23
Winn, Caroline Bodi; Artim, Stephen C; Jamiel, Morgan S et al. (2018) Lung Lobe Torsion in an Adult Male Common Marmoset (Callithrix jacchus). Comp Med 68:314-318
DiChiara, Andrew S; Li, Rasia C; Suen, Patreece H et al. (2018) A cysteine-based molecular code informs collagen C-propeptide assembly. Nat Commun 9:4206
Keegan, Caroline; Krutzik, Stephan; Schenk, Mirjam et al. (2018) Mycobacterium tuberculosis Transfer RNA Induces IL-12p70 via Synergistic Activation of Pattern Recognition Receptors within a Cell Network. J Immunol 200:3244-3258
Caron, Tyler J; Artim, Stephen C; Israelsen, William J et al. (2018) Cutaneous Dermatophilosis in a Meadow Jumping Mouse (Zapus hudsonius). Comp Med 68:25-30
Phillips, Angela M; Doud, Michael B; Gonzalez, Luna O et al. (2018) Enhanced ER proteostasis and temperature differentially impact the mutational tolerance of influenza hemagglutinin. Elife 7:
Wang, Xin; Garcia, Carlos T; Gong, Guanyu et al. (2018) Automated Online Solid-Phase Derivatization for Sensitive Quantification of Endogenous S-Nitrosoglutathione and Rapid Capture of Other Low-Molecular-Mass S-Nitrosothiols. Anal Chem 90:1967-1975
Moore, Christopher L; Papa 3rd, Louis J; Shoulders, Matthew D (2018) A Processive Protein Chimera Introduces Mutations across Defined DNA Regions In Vivo. J Am Chem Soc 140:11560-11564
Chan, Cheryl; Pham, Phuong; Dedon, Peter C et al. (2018) Lifestyle modifications: coordinating the tRNA epitranscriptome with codon bias to adapt translation during stress responses. Genome Biol 19:228

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