Abstract

The overall objective of the Animal Models and Pathology Facilities Core is to provide Center members with state-of-the-art pathology support, transgenic resources and a centrally-managed AAALAC-approved animal holding and surgical facility. The Core is staffed with experienced personnel and is equipped with essential equipment to generate genetically engineered mice (GEM), rederive imported mice by embryo transfer rederivation, provide colony management, and prepare and interpret tissue samples by histological and image analysis.
The specific aims are as follows: (1) To generate transgenic animals by pronuclear microinjection of DNA constructs or embryonic stem cell manipulation and provide colony management of GEM for use by Center members; (2) To rederive transgenic and other specialized mouse strains by embryo transfer to assure specific pathogen free status of all mice used in this program; (3) To provide histology, pathology, molecular characterization and imaging expertise for extensive phenotyping and assessment of pathological damage of GEM and other animal models as needed.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Center Core Grants (P30)
Project #
5P30ES002109-30
Application #
7798630
Study Section
Environmental Health Sciences Review Committee (EHS)
Project Start
Project End
Budget Start
2009-04-01
Budget End
2010-03-31
Support Year
30
Fiscal Year
2009
Total Cost
$316,291
Indirect Cost

  Institution

Name
Massachusetts Institute of Technology
Department
Type
DUNS #
001425594
City
Cambridge
State
MA
Country
United States
Zip Code
02139

  Publications

Wadduwage, Dushan N; Kay, Jennifer; Singh, Vijay Raj et al. (2018) Automated fluorescence intensity and gradient analysis enables detection of rare fluorescent mutant cells deep within the tissue of RaDR mice. Sci Rep 8:12108
Jackson, Megan N; Oh, Seokjoon; Kaminsky, Corey J et al. (2018) Strong Electronic Coupling of Molecular Sites to Graphitic Electrodes via Pyrazine Conjugation. J Am Chem Soc 140:1004-1010
Chen, Percival Yang-Ting; Funk, Michael A; Brignole, Edward J et al. (2018) Disruption of an oligomeric interface prevents allosteric inhibition of Escherichia coli class Ia ribonucleotide reductase. J Biol Chem 293:10404-10412
Lieberman, Mia T; Madden, Carolyn M; Ma, Eric J et al. (2018) Evaluation of 6 Methods for Aerobic Bacterial Sanitization of Smartphones. J Am Assoc Lab Anim Sci 57:24-29
Edington, Collin D; Chen, Wen Li Kelly; Geishecker, Emily et al. (2018) Interconnected Microphysiological Systems for Quantitative Biology and Pharmacology Studies. Sci Rep 8:4530
Mannion, Anthony; Shen, Zeli; Feng, Yan et al. (2018) Gamma-glutamyltranspeptidase expression by Helicobacter saguini, an enterohepatic Helicobacter species isolated from cotton top tamarins with chronic colitis. Cell Microbiol :e12968
Tajai, Preechaya; Fedeles, Bogdan I; Suriyo, Tawit et al. (2018) An engineered cell line lacking OGG1 and MUTYH glycosylases implicates the accumulation of genomic 8-oxoguanine as the basis for paraquat mutagenicity. Free Radic Biol Med 116:64-72
Neumann, Wilma; Nolan, Elizabeth M (2018) Evaluation of a reducible disulfide linker for siderophore-mediated delivery of antibiotics. J Biol Inorg Chem 23:1025-1036
Pereira, Gavin C; Sanchez, Laura; Schaughency, Paul M et al. (2018) Properties of LINE-1 proteins and repeat element expression in the context of amyotrophic lateral sclerosis. Mob DNA 9:35
Wang, Lianrong; Jiang, Susu; Deng, Zixin et al. (2018) DNA phosphorothioate modification - a new multi-functional epigenetic system in bacteria. FEMS Microbiol Rev :

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