This F&S Core will provide CEG members with the ability to develop transgenic mice and to bred the mice under close maintenance in a pathogen-free environment. The Core consists of facilities and personnel for: 1) making gain-of- function transgenic mice in which DNA is micro- injected into fertilized mouse eggs; 2) developing transgenic mice derived from gene-targeted embryonic stem cells in which a function is lost (knockout); and 3) carrying out selected breeding to generate desired combinations of genetic alteration or to modify the genetic background of transgenic mice for experimental use. Dr. John Duffy is the director of this F&S Core. The personnel responsible for the construction of gain-of-function mice and of KO mice is Jonathan Neumann and john Duffy, respectively. Dr. Tom Doetschman, a well known expert in the transgenic and gene knockout field who was instrumental in establishing this core, will continue to provide new ideas and approaches to the facility. Consultation will be provided to Center investigators free of charge. Technical services for construction and breeding of gain-of-function and kO mice will be provided on a fee-for-service basis paid for by funds allocated to this core. Gain-of-function mice will be produced at a cost of $2,500/construct, while production of KO mouse line will be at a cost of $9,500, this includes gene targeting in ES cell ($4,300) and microinjection of E? cells to produce gene targeted mice ($5,200). Costs for mouse breeding will be $2,275. Center investigators will first consult with J. Duffy an J Neumann to discuss their proposals for the generation of specific mice. If it is deemed a feasible project in these discussions, the investigators will consult with J. Duffy and J. Neumann on the construction of targeting vectors and the molecular biological aspects of the project. At this point, the Center investigators, J. Duffy, J. Neumann, and the Center Internal Advisory Board will meet to discuss the relevance for the proposed construction of the transgenic mice to the mission of the Center. It will be decided whether funds set aside for the generation of transgenic mice in the Center budget will be released to the Core to support the generation of the mice proposed.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Center Core Grants (P30)
Project #
2P30ES006096-06A1
Application #
6239647
Study Section
Project Start
1997-09-30
Project End
1998-03-31
Budget Start
1996-10-01
Budget End
1997-09-30
Support Year
6
Fiscal Year
1997
Total Cost
Indirect Cost
Name
University of Cincinnati
Department
Type
DUNS #
City
Cincinnati
State
OH
Country
United States
Zip Code
45221
Zhang, Hongmei; Yolton, Kimberly; Webster, Glenys M et al. (2018) Prenatal and childhood perfluoroalkyl substances exposures and children's reading skills at ages 5 and 8years. Environ Int 111:224-231
Sharma, Manju; Zhang, Xiang; Huang, Shouxiong (2018) Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking. J Vis Exp :
Vuong, Ann M; Braun, Joseph M; Webster, Glenys M et al. (2018) Polybrominated diphenyl ether (PBDE) exposures and thyroid hormones in children at age 3?years. Environ Int 117:339-347
Pugh, Amanda M; Giannini, Courtney M; Pinney, Susan M et al. (2018) Characteristics and diagnosis of pregnancy and lactation associated breast cancer: Analysis of a self-reported regional registry. Am J Surg 216:809-812
Brunst, Kelly J; Tignor, Nicole; Just, Allan et al. (2018) Cumulative lifetime maternal stress and epigenome-wide placental DNA methylation in the PRISM cohort. Epigenetics 13:665-681
Alexander, Jacqueline; Teague, April M; Chen, Jing et al. (2018) Offspring sex impacts DNA methylation and gene expression in placentae from women with diabetes during pregnancy. PLoS One 13:e0190698
Kim, Dasom; Chen, Zi; Zhou, Lin-Fu et al. (2018) Air pollutants and early origins of respiratory diseases. Chronic Dis Transl Med 4:75-94
Brunst, Kelly J; Sanchez-Guerra, Marco; Chiu, Yueh-Hsiu Mathilda et al. (2018) Prenatal particulate matter exposure and mitochondrial dysfunction at the maternal-fetal interface: Effect modification by maternal lifetime trauma and child sex. Environ Int 112:49-58
Isiugo, Kelechi; Newman, Nicholas; Jandarov, Roman et al. (2018) Assessing the accuracy of commercially available gas sensors for the measurement of ambient ozone and nitrogen dioxide. J Occup Environ Hyg 15:782-791
Li, Tao; Hu, Rong; Chen, Zi et al. (2018) Fine particulate matter (PM2.5): The culprit for chronic lung diseasesĀ in China. Chronic Dis Transl Med 4:176-186

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