The goals of the Morphology &Microscopic Imaging Module are to make available to vision researchers the instruments and expertise required (1) to perform microscopic analyses of ocular specimens, subcellular fractions, or cultured cells, and (2) to generate images of microscopic preparations for analysis, publication, presentation and display. The heart of the Module is a centralized facility for basic microscopy where tissues are processed and blocks are sectioned. This is essential for our group since virtually all our lab scientists must examine cell or tissue structure for some aspects of their work, but individual investigators cannot justify or support a fully-equipped microscopy lab -- or the ongoing salaries of skilled microscopy technicians. In addition to supporting tissue processing, a key service of the Module is to facilitate access to institutional centers for electron microscopy and confocal imaging. This latter activity is in keeping with the overall strategy for our Core program emphasized in this budget cycle: to use our Modules to help investigators take advantage of institutional research resources thereby enhancing productivity by providing access to costly instruments that could not be individually supported while integrating our Core vision group with the broader research community on campus.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
5P30EY001931-37
Application #
8502506
Study Section
Special Emphasis Panel (ZEY1-VSN)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
37
Fiscal Year
2013
Total Cost
$98,896
Indirect Cost
$34,258
Name
Medical College of Wisconsin
Department
Type
DUNS #
937639060
City
Milwaukee
State
WI
Country
United States
Zip Code
53226
Hirji, Nashila; Georgiou, Michalis; Kalitzeos, Angelos et al. (2018) Longitudinal Assessment of Retinal Structure in Achromatopsia Patients With Long-Term Follow-up. Invest Ophthalmol Vis Sci 59:5735-5744
Krawitz, Brian D; Phillips, Erika; Bavier, Richard D et al. (2018) Parafoveal Nonperfusion Analysis in Diabetic Retinopathy Using Optical Coherence Tomography Angiography. Transl Vis Sci Technol 7:4
Sajdak, Benjamin S; Bell, Brent A; Lewis, Tylor R et al. (2018) Assessment of Outer Retinal Remodeling in the Hibernating 13-Lined Ground Squirrel. Invest Ophthalmol Vis Sci 59:2538-2547
Datta, Shyamtanu; Renwick, Marian; Chau, Viet Q et al. (2018) A Destabilizing Domain Allows for Fast, Noninvasive, Conditional Control of Protein Abundance in the Mouse Eye - Implications for Ocular Gene Therapy. Invest Ophthalmol Vis Sci 59:4909-4920
Mainali, Laxman; O'Brien, William J; Subczynski, Witold K (2018) Detection of cholesterol bilayer domains in intact biological membranes: Methodology development and its application to studies of eye lens fiber cell plasma membranes. Exp Eye Res 178:72-81
Hendee, Kathryn E; Sorokina, Elena A; Muheisen, Sanaa S et al. (2018) PITX2 deficiency and associated human disease: insights from the zebrafish model. Hum Mol Genet 27:1675-1695
Lapierre-Landry, Maryse; Huckenpahler, Alison L; Link, Brian A et al. (2018) Imaging Melanin Distribution in the Zebrafish Retina Using Photothermal Optical Coherence Tomography. Transl Vis Sci Technol 7:4
Patterson, Emily J; Kalitzeos, Angelos; Kasilian, Melissa et al. (2018) Residual Cone Structure in Patients With X-Linked Cone Opsin Mutations. Invest Ophthalmol Vis Sci 59:4238-4248
Davidson, Benjamin; Kalitzeos, Angelos; Carroll, Joseph et al. (2018) Automatic Cone Photoreceptor Localisation in Healthy and Stargardt Afflicted Retinas Using Deep Learning. Sci Rep 8:7911
Reid, Christopher A; Nettesheim, Emily R; Connor, Thomas B et al. (2018) Development of an inducible anti-VEGF rAAV gene therapy strategy for the treatment of wet AMD. Sci Rep 8:11763

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