The Molecular Biology Module provides services involved with the production of transgenic, knock-out and knock-in mice and construction of gene constructs used for an array of applications (e.g., in situ hybridization probes), preparation of DNA clones and probes, and consultation or direct service for the design of polymerase chain reaction primers and optimization of reaction conditions. The module also provides limited- scale DNA sequencing, consultation or direct services for phosphorimaging and flow cytometry for cell analysis, and maintenance and oversight of shared molecular biology instrumentation. The module occupies a new laboratory designed for its exclusive use. The module is staffed by two full-time molecular biology technicians with advanced skills in molecular cloning, DNA manipulations, animal husbandry and microinjection. The services of this module enhance the environment for research by making molecular biology an accessible tool for any member of the vision core group. The environment is further enhanced by providing training and support in the use of modern shared instrumentation for molecular biology, including the STORM phosphorimager and Beckman Coulter FC500 flow cytometer.
| Lin, Jonathan B; Apte, Rajendra S (2018) NAD+ and sirtuins in retinal degenerative diseases: A look at future therapies. Prog Retin Eye Res 67:118-129 |
| Porter, Lane C; Franczyk, Michael P; Pietka, Terri et al. (2018) NAD+-dependent deacetylase SIRT3 in adipocytes is dispensable for maintaining normal adipose tissue mitochondrial function and whole body metabolism. Am J Physiol Endocrinol Metab 315:E520-E530 |
| De Maria, Alicia; Zhao, Haiqing; Bassnett, Steven (2018) Expression of potassium-dependent sodium-calcium exchanger in the murine lens. Exp Eye Res 167:18-24 |
| Lin, Joseph B; Sene, Abdoulaye; Wiley, Luke A et al. (2018) WNT7A/B promote choroidal neovascularization. Exp Eye Res 174:107-112 |
| Kiser, Philip D; Zhang, Jianye; Sharma, Aditya et al. (2018) Retinoid isomerase inhibitors impair but do not block mammalian cone photoreceptor function. J Gen Physiol 150:571-590 |
| Soto, Florentina; Zhao, Lei; Kerschensteiner, Daniel (2018) Synapse maintenance and restoration in the retina by NGL2. Elife 7: |
| Ban, Norimitsu; Siegfried, Carla J; Apte, Rajendra S (2018) Monitoring Neurodegeneration in Glaucoma: Therapeutic Implications. Trends Mol Med 24:7-17 |
| Ban, Norimitsu; Lee, Tae Jun; Sene, Abdoulaye et al. (2018) Disrupted cholesterol metabolism promotes age-related photoreceptor neurodegeneration. J Lipid Res 59:1414-1423 |
| Buckingham, Erin M; Foley, Maria A; Grose, Charles et al. (2018) Identification of Herpes Zoster-Associated Temporal Arteritis Among Cases of Giant Cell Arteritis. Am J Ophthalmol 187:51-60 |
| Stunkel, Leanne; Kung, Nathan H; Wilson, Bradley et al. (2018) Incidence and Causes of Overdiagnosis of Optic Neuritis. JAMA Ophthalmol 136:76-81 |
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