The Molecular Biology Module provides services involved with the production of transgenic, knock-out and knock-in mice and construction of gene constructs used for an array of applications (e.g., in situ hybridization probes), preparation of DNA clones and probes, and consultation or direct service for the design of polymerase chain reaction primers and optimization of reaction conditions. The module also provides limited- scale DNA sequencing, consultation or direct services for phosphorimaging and flow cytometry for cell analysis, and maintenance and oversight of shared molecular biology instrumentation. The module occupies a new laboratory designed for its exclusive use. The module is staffed by two full-time molecular biology technicians with advanced skills in molecular cloning, DNA manipulations, animal husbandry and microinjection. The services of this module enhance the environment for research by making molecular biology an accessible tool for any member of the vision core group. The environment is further enhanced by providing training and support in the use of modern shared instrumentation for molecular biology, including the STORM phosphorimager and Beckman Coulter FC500 flow cytometer.

National Institute of Health (NIH)
National Eye Institute (NEI)
Center Core Grants (P30)
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Special Emphasis Panel (ZEY1)
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Washington University
Saint Louis
United States
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Hamilton, Paul D; Andley, Usha P (2018) In vitro interactions of histones and ?-crystallin. Biochem Biophys Rep 15:7-12
Lin, Jonathan B; Moolani, Harsh V; Sene, Abdoulaye et al. (2018) Macrophage microRNA-150 promotes pathological angiogenesis as seen in age-related macular degeneration. JCI Insight 3:
Kraus, Courtney L; Culican, Susan M (2018) New advances in amblyopia therapy II: refractive therapies. Br J Ophthalmol 102:1611-1614
Ban, Norimitsu; Lee, Tae Jun; Sene, Abdoulaye et al. (2018) Impaired monocyte cholesterol clearance initiates age-related retinal degeneration and vision loss. JCI Insight 3:
Lin, Jonathan B; Kubota, Shunsuke; Mostoslavsky, Raul et al. (2018) Role of Sirtuins in Retinal Function Under Basal Conditions. Adv Exp Med Biol 1074:561-567
Vinberg, Frans; Chen, Jeannie; Kefalov, Vladimir J (2018) Regulation of calcium homeostasis in the outer segments of rod and cone photoreceptors. Prog Retin Eye Res 67:87-101
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Lin, Jonathan B; Sene, Abdoulaye; Santeford, Andrea et al. (2018) Oxysterol Signatures Distinguish Age-Related Macular Degeneration from Physiologic Aging. EBioMedicine 32:9-20

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