The Molecular Biology Module provides services involved with the production of transgenic, knock-out and knock-in mice and construction of gene constructs used for an array of applications (e.g., in situ hybridization probes), preparation of DNA clones and probes, and consultation or direct service for the design of polymerase chain reaction primers and optimization of reaction conditions. The module also provides limited- scale DNA sequencing, consultation or direct services for phosphorimaging and flow cytometry for cell analysis, and maintenance and oversight of shared molecular biology instrumentation. The module occupies a new laboratory designed for its exclusive use. The module is staffed by two full-time molecular biology technicians with advanced skills in molecular cloning, DNA manipulations, animal husbandry and microinjection. The services of this module enhance the environment for research by making molecular biology an accessible tool for any member of the vision core group. The environment is further enhanced by providing training and support in the use of modern shared instrumentation for molecular biology, including the STORM phosphorimager and Beckman Coulter FC500 flow cytometer.

National Institute of Health (NIH)
National Eye Institute (NEI)
Center Core Grants (P30)
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Special Emphasis Panel (ZEY1)
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Washington University
Saint Louis
United States
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Vinberg, Frans; Kefalov, Vladimir J (2018) Investigating the Ca2+-dependent and Ca2+-independent mechanisms for mammalian cone light adaptation. Sci Rep 8:15864
Lin, Jonathan B; Sene, Abdoulaye; Santeford, Andrea et al. (2018) Oxysterol Signatures Distinguish Age-Related Macular Degeneration from Physiologic Aging. EBioMedicine 32:9-20
Johnson, Keith P; Zhao, Lei; Kerschensteiner, Daniel (2018) A Pixel-Encoder Retinal Ganglion Cell with Spatially Offset Excitatory and Inhibitory Receptive Fields. Cell Rep 22:1462-1472
Zhou, Yuefang; Shiels, Alan (2018) Epha2 and Efna5 participate in lens cell pattern-formation. Differentiation 102:1-9
Lin, Jonathan B; Apte, Rajendra S (2018) NAD+ and sirtuins in retinal degenerative diseases: A look at future therapies. Prog Retin Eye Res 67:118-129
Porter, Lane C; Franczyk, Michael P; Pietka, Terri et al. (2018) NAD+-dependent deacetylase SIRT3 in adipocytes is dispensable for maintaining normal adipose tissue mitochondrial function and whole body metabolism. Am J Physiol Endocrinol Metab 315:E520-E530
De Maria, Alicia; Zhao, Haiqing; Bassnett, Steven (2018) Expression of potassium-dependent sodium-calcium exchanger in the murine lens. Exp Eye Res 167:18-24
Lin, Joseph B; Sene, Abdoulaye; Wiley, Luke A et al. (2018) WNT7A/B promote choroidal neovascularization. Exp Eye Res 174:107-112
Kiser, Philip D; Zhang, Jianye; Sharma, Aditya et al. (2018) Retinoid isomerase inhibitors impair but do not block mammalian cone photoreceptor function. J Gen Physiol 150:571-590
Soto, Florentina; Zhao, Lei; Kerschensteiner, Daniel (2018) Synapse maintenance and restoration in the retina by NGL2. Elife 7:

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