of the core: The Tc/Mol core, located in dedicated space in the Department of Anatomy/Cell Biology, provides a centralized facility for maintenance and availability of immortalized cell lines and assistance with tissue and organ culture. In addition, the core will continue to assist investigators in the use of various techniques in molecular biology. [New to the core is the addition of virus production capability, which will include assistance on HEK cell line culture for AAV vector packaging and purification, spearheaded by Dr. Pan, the core director]. An experienced research assistant, Kerry Vistisen, will be available to assist investigators and their staff who have little expertise in these areas to develop experimental methods relevant to their studies. In addition, she will assist investigators with performance of molecular and other (Western blot) techniques. Projects proposed requiring core use will be presented to the core director (Dr. Pan) for consultation. Once approved, the projects will be prioritized, with NEI R01 funded projects given priority and undertaken by the core research assistant. This may involve performance of the task, or training of personnel-or both. If two projects require similar personnel training, for example, the research assistant will consolidate training to be most efficient whenever possible. Space and equipment for molecular studies have also expanded and these changes have enhanced the use of this core. In fact, R01 funded investigators who lack experience in the technology required for molecular biology, tissue/organ culture, and/or virus production will still rely heavily on the equipment and availability of the research assistant for technical advice or hands on assistance for their projects. Those knowledgeable in the technical aspects involved will use the facility to enhance their own studies and to begin pilot or collaborative research projects. This centralized core will thus continue to provide a reasonable, cost efficient mechanism to facilitate and enhance numerous NEI R0l funded research projects as its top priority. It is also the goal of the core to enhance, at lower priority, collaboration and productivity of those who are performing pilot studies in vision, by providing some financial offset (See letter of support. Dr. Stanton and Administrative core) which will encourage interaction with scientists from diverse fields, with those in vision and potentially attract them to vision research.

National Institute of Health (NIH)
National Eye Institute (NEI)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZEY1-VSN (03))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Wayne State University
United States
Zip Code
Curtiss, Elizabeth; Jiang, Youde; Liu, Li et al. (2018) Epac1 Restores Normal Insulin Signaling through a Reduction in Inflammatory Cytokines. Mediators Inflamm 2018:3809092
Singh, Pawan Kumar; Khatri, Indu; Jha, Alokkumar et al. (2018) Determination of system level alterations in host transcriptome due to Zika virus (ZIKV) Infection in retinal pigment epithelium. Sci Rep 8:11209
Han, Jing; Li, Yue; Liu, Xiuli et al. (2018) Metformin suppresses retinal angiogenesis and inflammation in vitro and in vivo. PLoS One 13:e0193031
Berkowitz, Bruce A; Podolsky, Robert H; Berri, Ali M et al. (2018) Dark Rearing Does Not Prevent Rod Oxidative Stress In Vivo in Pde6brd10 Mice. Invest Ophthalmol Vis Sci 59:1659-1665
Jiang, Youde; Liu, Li; Steinle, Jena J (2018) miRNA15a regulates insulin signal transduction in the retinal vasculature. Cell Signal 44:28-32
Liu, Li; Patel, Paragi; Steinle, Jena J (2018) PKA regulates HMGB1 through activation of IGFBP-3 and SIRT1 in human retinal endothelial cells cultured in high glucose. Inflamm Res 67:1013-1019
Carion, Thomas W; Greenwood, Matthew; Ebrahim, Abdul Shukkur et al. (2018) Immunoregulatory role of 15-lipoxygenase in the pathogenesis of bacterial keratitis. FASEB J 32:5026-5038
Shi, Haoshen; Berger, Elizabeth A (2018) Characterization of Site-Specific Phosphorylation of NF-?B p65 in Retinal Cells in Response to High Glucose and Cytokine Polarization. Mediators Inflamm 2018:3020675
Guest, John M; Singh, Pawan Kumar; Revankar, Sanjay G et al. (2018) Isavuconazole for Treatment of Experimental Fungal Endophthalmitis Caused by Aspergillus fumigatus. Antimicrob Agents Chemother 62:
Berkowitz, Bruce A; Podolsky, Robert H; Qian, Haohua et al. (2018) Mitochondrial Respiration in Outer Retina Contributes to Light-Evoked Increase in Hydration In Vivo. Invest Ophthalmol Vis Sci 59:5957-5964

Showing the most recent 10 out of 357 publications