Abstract

? Flow Cytometry Module Flow cytometry is a powerful technology for multiparameter analysis of single cells contained within a mixed cell suspension. Within minutes, millions of cells labeled with fluorescent antibodies or dyes pass through laser beams that generate signals which reflect cell morphology and fluorescent intensity measurements. Sorting of up to four cell populations with desired phenotypes defined by up to 15 different parameters is also possible. The overall goal of the Flow Cytometry Module is to allow vision scientists to easily utilize flow cytometric applications in their research, and to lower the barrier to developing and utilizing flow cytometric applications in new projects. !

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
5P30EY008098-32
Application #
9921436
Study Section
Special Emphasis Panel (ZEY1)
Project Start
Project End
Budget Start
2020-04-01
Budget End
2021-03-31
Support Year
32
Fiscal Year
2020
Total Cost
Indirect Cost

  Institution

Name
University of Pittsburgh
Department
Type
DUNS #
004514360
City
Pittsburgh
State
PA
Country
United States
Zip Code
15260

  Publications

Avery, Lyndsay; Filderman, Jessica; Szymczak-Workman, Andrea L et al. (2018) Tim-3 co-stimulation promotes short-lived effector T cells, restricts memory precursors, and is dispensable for T cell exhaustion. Proc Natl Acad Sci U S A 115:2455-2460
Yang, Xiao-Ling; van der Merwe, Yolandi; Sims, Jeffrey et al. (2018) Age-related Changes in Eye, Brain and Visuomotor Behavior in the DBA/2J Mouse Model of Chronic Glaucoma. Sci Rep 8:4643
Dang, Yalong; Waxman, Susannah; Wang, Chao et al. (2018) Intraocular pressure elevation precedes a phagocytosis decline in a model of pigmentary glaucoma. F1000Res 7:174
Romanowski, Eric G; Stella, Nicholas A; Yates, Kathleen A et al. (2018) In Vitro Evaluation of a Hypochlorous Acid Hygiene Solution on Established Biofilms. Eye Contact Lens 44 Suppl 2:S187-S191
Snyder, Adam C; Issar, Deepa; Smith, Matthew A (2018) What does scalp electroencephalogram coherence tell us about long-range cortical networks? Eur J Neurosci 48:2466-2481
Tran, Huong; Wallace, Jacob; Zhu, Ziyi et al. (2018) Seeing the Hidden Lamina: Effects of Exsanguination on the Optic Nerve Head. Invest Ophthalmol Vis Sci 59:2564-2575
Huang, Ge; Ramachandran, Suchitra; Lee, Tai Sing et al. (2018) Neural Correlate of Visual Familiarity in Macaque Area V2. J Neurosci 38:8967-8975
Garcia, Carlos J; Pericleous, Androulla; Elsayed, Mennat et al. (2018) Serralysin family metalloproteases protects Serratia marcescens from predation by the predatory bacteria Micavibrio aeruginosavorus. Sci Rep 8:14025
Kumar, Ajay; Xu, Yi; Yang, Enzhi et al. (2018) Stemness and Regenerative Potential of Corneal Stromal Stem Cells and Their Secretome After Long-Term Storage: Implications for Ocular Regeneration. Invest Ophthalmol Vis Sci 59:3728-3738
Dang, Yalong; Waxman, Susannah; Wang, Chao et al. (2018) A porcine ex vivo model of pigmentary glaucoma. Sci Rep 8:5468

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