Abstract

Module 3. Molecular Biology and Genotyping (MBG) Dr. Palczewski, Director, Ganna Yakubenko, MS, Manager Overview and Resources: The Molecular Biology Module has been in effect since the flrst funding period of the Case VSRC P30 grant in 1997. In the most recent funding period, the Module has worked especially closely with the Specialized Animal Resource Module to genotype established mouse strains and to characterize newly generated strains using the Cre-Lox recombinase system. Although genotyping is the predominant function of this Module, it also comprises a BioRad Versa Doc Imaging Systemc and a new Eppendorf Realplex2 Master Cycler qPCR machine in addition to the Applied Bioscience qPCR thermocycler, all of which are extensively used by VSRC investigators. Overall, we anticipate that the Module will continue to be very heavily utilized. The MBG Module is located in the centralized VSRC facilities in the Institute of Pathology, and provides technical expertise, access to equipment and techniques for PCR analysis and genotyping, quantitative PCR, and imaging of agarose and acrylamide gels.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
5P30EY011373-18
Application #
8734428
Study Section
Special Emphasis Panel (ZEY1)
Project Start
Project End
Budget Start
2014-09-01
Budget End
2015-08-31
Support Year
18
Fiscal Year
2014
Total Cost
Indirect Cost

  Institution

Name
Case Western Reserve University
Department
Type
DUNS #
City
Cleveland
State
OH
Country
United States
Zip Code
44106

  Publications

Hughes, Taylor E T; Pumroy, Ruth A; Yazici, Aysenur Torun et al. (2018) Structural insights on TRPV5 gating by endogenous modulators. Nat Commun 9:4198
Gulati, Sahil; Jin, Hui; Masuho, Ikuo et al. (2018) Targeting G protein-coupled receptor signaling at the G protein level with a selective nanobody inhibitor. Nat Commun 9:1996
Senapati, Subhadip; Gragg, Megan; Samuels, Ivy S et al. (2018) Effect of dietary docosahexaenoic acid on rhodopsin content and packing in photoreceptor cell membranes. Biochim Biophys Acta Biomembr 1860:1403-1413
Cheng, Yan; Du, Yunpeng; Liu, Haitao et al. (2018) Photobiomodulation Inhibits Long-term Structural and Functional Lesions of Diabetic Retinopathy. Diabetes 67:291-298
Flückiger, Rudolf; Cocuzzi, Enzo; Nagaraj, Ram H et al. (2018) DAF in diabetic patients is subject to glycation/inactivation at its active site residues. Mol Immunol 93:246-252
Lam, Morrie; Mast, Natalia; Pikuleva, Irina A (2018) Drugs and Scaffold That Inhibit Cytochrome P450 27A1 In Vitro and In Vivo. Mol Pharmacol 93:101-108
Gragg, Megan; Park, Paul S-H (2018) Misfolded rhodopsin mutants display variable aggregation properties. Biochim Biophys Acta Mol Basis Dis 1864:2938-2948
Parmar, Vipul M; Parmar, Tanu; Arai, Eisuke et al. (2018) A2E-associated cell death and inflammation in retinal pigmented epithelial cells from human induced pluripotent stem cells. Stem Cell Res 27:95-104
Cheng, Yu-Shiuan; Linetsky, Mikhail; Gu, Xilin et al. (2018) Light-induced generation and toxicity of docosahexaenoate-derived oxidation products in retinal pigmented epithelial cells. Exp Eye Res :
Palczewska, Grazyna; Stremplewski, Patrycjusz; Suh, Susie et al. (2018) Two-photon imaging of the mammalian retina with ultrafast pulsing laser. JCI Insight 3:

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