The goal of the Histology, Microscopy, and Imaging Module is to enhance the capabilities of individual vision research laboratories by enabling them to conduct studies that require understanding the ocular tissue function at the cellular level. This goal is accomplished by providing the infrastructure for two types of services - histology and imaging. The Histology Facility of the Module prepares in a timely fashion cryo or paraffin sections of eyes and mounts them on slides for histo/immunohistochemistry analyses or laser capture microdissection. This Facility also conducts routine histological stains. The Microscopy Facility of the Module helps investigators to produce publication quality images or videos from histological slides, fixed cells, live cells or in vivo mouse eyes. A physical space, resources, and expertise of the Module, operated and supervised by highly experienced personnel, serve as the basis for the following Specific Aims: 1) to process, embed, and section eyes followed by section mounting on slides and staining (if requested); 2) to guide users in the proper dissection, fixation and staining techniques, thus ensuring the production of high quality histological specimens; 3) to provide access to Module's microscopes along with training and consultation in all aspects of a microscopy-based experiments; 4) to provide access to and training on the use of Metamorph imaging software to properly analyze and generate relevant data from images; 5) to assist in production of publication quality images and/or videos. The Histology, Microscopy & Imaging Module has the heaviest usage of all P30 Core Grant Modules and will continue assisting VSRC investigators in producing high-quality and high-profile vision-related publications.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
5P30EY011373-24
Application #
10001526
Study Section
Special Emphasis Panel (ZEY1)
Project Start
1997-04-01
Project End
2022-08-31
Budget Start
2020-09-01
Budget End
2021-08-31
Support Year
24
Fiscal Year
2020
Total Cost
Indirect Cost
Name
Case Western Reserve University
Department
Type
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106
Basak, Sandip; Gicheru, Yvonne; Samanta, Amrita et al. (2018) Cryo-EM structure of 5-HT3A receptor in its resting conformation. Nat Commun 9:514
Mamidi, Ranganath; Li, Jiayang; Doh, Chang Yoon et al. (2018) Impact of the Myosin Modulator Mavacamten on Force Generation and Cross-Bridge Behavior in a Murine Model of Hypercontractility. J Am Heart Assoc 7:e009627
Basak, Sandip; Gicheru, Yvonne; Rao, Shanlin et al. (2018) Cryo-EM reveals two distinct serotonin-bound conformations of full-length 5-HT3A receptor. Nature 563:270-274
Gao, Songqi; Parmar, Tanu; Palczewska, Grazyna et al. (2018) Protective Effect of a Locked Retinal Chromophore Analog against Light-Induced Retinal Degeneration. Mol Pharmacol 94:1132-1144
Malemud, Charles J (2018) The role of the JAK/STAT signal pathway in rheumatoid arthritis. Ther Adv Musculoskelet Dis 10:117-127
Clark, Heather L; Minns, Martin S; Sun, Yan et al. (2018) Atovaquone Impairs Growth of Aspergillus and Fusarium Keratitis Isolates by Modulating Mitochondrial Function and Zinc Homeostasis. Invest Ophthalmol Vis Sci 59:1589-1598
Parmar, Tanu; Parmar, Vipul M; Perusek, Lindsay et al. (2018) Lipocalin 2 Plays an Important Role in Regulating Inflammation in Retinal Degeneration. J Immunol 200:3128-3141
Widjaja-Adhi, Made Airanthi K; Ramkumar, Srinivasagan; von Lintig, Johannes (2018) Protective role of carotenoids in the visual cycle. FASEB J :fj201800467R
Chen, Yuanyuan; Chen, Yu; Jastrzebska, Beata et al. (2018) A novel small molecule chaperone of rod opsin and its potential therapy for retinal degeneration. Nat Commun 9:1976
Mast, Natalia; Bederman, Ilya R; Pikuleva, Irina A (2018) Retinal Cholesterol Content Is Reduced in Simvastatin-Treated Mice Due to Inhibited Local Biosynthesis Albeit Increased Uptake of Serum Cholesterol. Drug Metab Dispos 46:1528-1537

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