-SPECTROSCOPIC AND BIOIMAGING CORE The Redox Biology Center's (RBC's) Spectroscopy and Bioimaging Core (Core B) provides an array of tools for investigators to acquire quantitative descriptions of protein structure and dynamics, kinetics, ligand binding, metal analysis, and interactions with other biological macromolecules. The Core, as evidenced by the fact it provided support for numerous extramural grant awards and contributed to 179 publications by RBC members during the Phase I and II funding cycles, has had a tremendous impact on the research output of the Center. The Spectroscopy and Bioimaging Core is comprised of three main service branches that support RBC investigators and their collaborators, as well as other academic and industrial researchers across Nebraska and the region. The three components of the Core are: 1) instrumentation for studies of protein structure, dynamics, thermodynamics, kinetics, and elemental analysis;2) microscopy bioimaging;and 3) electron paramagnetic resonance (EPR) spectroscopy. This proposal request funds to support the Spectroscopy and Bioimaging Core toward achieving its Phase III aims, which are to: 1) maintain and provide state-of-the-art spectroscopy and bioimaging instrumentation and technical services to RBC investigators and non-RBC members and investigators outside the University of Nebraska system;2) provide preliminary data and analysis to assist in the success of RBC grant proposal submissions and train/educate RBC faculty and students in spectroscopic and bioimaging methods;and 3) facilitate research collaborations between RBC investigators and non-RBC scientists within (as well as outside of) the University of Nebraska system. During Phase II, the RBC significantly increased the research capacity in the Core, which has moved the RBC forward on a trajectory toward become a self-sustainable Center. The momentum of the Spectroscopy and Bioimaging Core has largely been sustained through major institutional support, which has enabled the RBC to acquire new equipment such as the inductively coupled plasma mass spectrometer, stopped-flow kinetics instrument, and EPR spectrometer. Long-term sustainability of the Core will be achieved by: 1) maintaining a substantial academic user base of well-funded investigators;2) supporting strategic collaborations and providing services to industry;and 3) through continued institutional support.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZRR1-RI-B (01))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Nebraska Lincoln
United States
Zip Code
Leiferman, Amy; Shu, Jiang; Grove, Ryan et al. (2018) A diet defined by its content of bovine milk exosomes and their RNA cargos has moderate effects on gene expression, amino acid profiles and grip strength in skeletal muscle in C57BL/6 mice. J Nutr Biochem 59:123-128
Catazaro, Jonathan; Andrews, Tessa; Milkovic, Nicole M et al. (2018) 15N CEST data and traditional model-free analysis capture fast internal dynamics of DJ-1. Anal Biochem 542:24-28
Dickinson, John D; Sweeter, Jenea M; Warren, Kristi J et al. (2018) Autophagy regulates DUOX1 localization and superoxide production in airway epithelial cells during chronic IL-13 stimulation. Redox Biol 14:272-284
Garza-Lombó, Carla; Schroder, Annika; Reyes-Reyes, Elsa M et al. (2018) mTOR/AMPK signaling in the brain: Cell metabolism, proteostasis and survival. Curr Opin Toxicol 8:102-110
Yuan, Sai; Sharma, Anuj Kumar; Richart, Alexandria et al. (2018) CHCA-1 is a copper-regulated CTR1 homolog required for normal development, copper accumulation, and copper-sensing behavior in Caenorhabditis elegans. J Biol Chem 293:10911-10925
Gardner, Stewart G; Marshall, Darrell D; Daum, Robert S et al. (2018) Metabolic Mitigation of Staphylococcus aureus Vancomycin Intermediate-Level Susceptibility. Antimicrob Agents Chemother 62:
Germany, Edward M; Zahayko, Nataliya; Huebsch, Mason L et al. (2018) The AAA ATPase Afg1 preserves mitochondrial fidelity and cellular health by maintaining mitochondrial matrix proteostasis. J Cell Sci 131:
Liu, Li-Kai; Becker, Donald F; Tanner, John J (2017) Structure, function, and mechanism of proline utilization A (PutA). Arch Biochem Biophys 632:142-157
Moxley, Michael A; Zhang, Lu; Christgen, Shelbi et al. (2017) Identification of a Conserved Histidine As Being Critical for the Catalytic Mechanism and Functional Switching of the Multifunctional Proline Utilization A Protein. Biochemistry 56:3078-3088
Korasick, David A; Gamage, Thameesha T; Christgen, Shelbi et al. (2017) Structure and characterization of a class 3B proline utilization A: Ligand-induced dimerization and importance of the C-terminal domain for catalysis. J Biol Chem 292:9652-9665

Showing the most recent 10 out of 109 publications