The COBRE Phase I and II awards supported the creation of a state-of-the-art facility to provide the research infrastructure for all Center investigators encompassing molecular biology, quantitative imaging, immunohistochemistry, genomics and RIA of angiotensin peptides and provided most of the resources available in our state-of-the-art facilities. One of these facilities is the Molecular, Imaging, and Analytical Core Facility which provides a comprehensive service for molecular biology, imaging and biochemistry support. The core houses instruments and equipment needed to perform advanced molecular biology, semiquantitative immunohistochemistry and bio-analytical experiments. Among the instruments used by the Core are the real-time PCR machines, automatic BioRobot for RNA extraction, ELISA, microplate reader with fluorescence and bioluminescence capabilities, a flow cytometer, an automatic immunostainer, luminometer, gammacounter, multiprobe robotic liquid handling system for large scale RIA assays, an upright and two upright and one inverted fluorescent high resolution microscopes coupled to digital cameras and software for imaging capturing and analyses, and a fully functional tissue culture facility licensed for viral work. In addition, the core is also equipped with modern equipment for HPLC separation and analysis of angiotensin peptides which combined with RIA provides a means for quantitative analysis of angiotensin peptides and other hormonal factors in plasma, tissues and urine. The Molecular, Imaging, and Analytical Core facility has contributed greatly to the research projects conducted by the investigators of the Tulane Hypertension and Renal Center and has supported training of junior faculty, postdoctoral fellows, graduate and medical students in the areas of physiology, hypertension and cardiovascular and renal diseases. These outcomes have translated to a significant increase in the number of papers published in peer reviewed journals and have contributed to competitiveness for NIH and other agencies funded by our center members. During the past 9 years, over 350 papers supported by COBRE were published, including 110 papers since 2010.

Public Health Relevance

The COBRE Phase I and II awards supported the creation of a state-of-the-art facility to provide research infrastructure for all THRCE investigators. Modern research projects related to hypertension and related diseases often require the analysis of many factors that are altered by the hypertension process. This core facility provides the investigators the tools for evaluating the molecular, biochemical and structural mechanisms responsible for this pathological process.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Center Core Grants (P30)
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Tulane University
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Cedillo-Couvert, Esteban A; Ricardo, Ana C; Chen, Jinsong et al. (2018) Self-reported Medication Adherence and CKD Progression. Kidney Int Rep 3:645-651
Liu, Hongbing; Chen, Shaowei; Yao, Xiao et al. (2018) Histone deacetylases 1 and 2 regulate the transcriptional programs of nephron progenitors and renal vesicles. Development 145:
Grams, Morgan E; Sang, Yingying; Ballew, Shoshana H et al. (2018) Predicting timing of clinical outcomes in patients with chronic kidney disease and severely decreased glomerular filtration rate. Kidney Int 93:1442-1451
Dobre, Mirela; Gaussoin, Sarah A; Bates, Jeffrey T et al. (2018) Serum Bicarbonate Concentration and Cognitive Function in Hypertensive Adults. Clin J Am Soc Nephrol 13:596-603
Shapiro, Brian P; Ambrosius, Walter T; Blackshear, Joseph L et al. (2018) Impact of Intensive Versus Standard Blood Pressure Management by Tertiles of Blood Pressure in SPRINT (Systolic Blood Pressure Intervention Trial). Hypertension 71:1064-1074
Rocco, Michael V; Sink, Kaycee M; Lovato, Laura C et al. (2018) Effects of Intensive Blood Pressure Treatment on Acute Kidney Injury Events in the Systolic Blood Pressure Intervention Trial (SPRINT). Am J Kidney Dis 71:352-361
Beddhu, Srinivasan; Greene, Tom; Boucher, Robert et al. (2018) Intensive systolic blood pressure control and incident chronic kidney disease in people with and without diabetes mellitus: secondary analyses of two randomised controlled trials. Lancet Diabetes Endocrinol 6:555-563
Anderson, Christopher E; Hamm, L Lee; Batuman, Gem et al. (2018) The association of angiogenic factors and chronic kidney disease. BMC Nephrol 19:117
Lightell Jr, Daniel J; Moss, Stephanie C; Woods, T Cooper (2018) Upregulation of miR-221 and -222 in response to increased extracellular signal-regulated kinases 1/2 activity exacerbates neointimal hyperplasia in diabetes mellitus. Atherosclerosis 269:71-78
Dungan, Kathleen; Craven, Timothy E; Soe, Kyaw et al. (2018) Influence of metabolic syndrome and race on the relationship between intensive blood pressure control and cardiovascular outcomes in the SPRINT cohort. Diabetes Obes Metab 20:629-637

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