The primary aims of the NMR core are to provide infrastructure and support for structural analysis of target macromolecules, their mutual interactions and with candidate drug molecules obtained from in silico searches and optimization and metabolomics of cancer cells and tissue. This includes stable isotope labeling strategies including N-15 and C-13 precursors. Our goals continue to be to streamline both data collection and analysis as well as introduce new labeling strategies to provide more detailed information about specific metabolic pathways, via Stable Isotope Resolved Metabolomics (SIRM). This includes NMR structural and biophysical analysis of macromolecules and their interactions with small molecules, as well as extensive use of the metabolomics capabilities of the Core as a part of the drug discovery/development process. A large fraction of research projects that use the facility are translational, using purified components, as well as animal models and human subjects. The NMR-based studies operate in tandem with activates of other Cores, especially the Modeling Core and, in the future, with the Transgenic Mouse Core. NMR is one of the few techniques that provides localized imaging and high resolution, high information content spectroscopy in vivo. This will be a major new direction of the Core. The overall goals of the Core will be achieved via the following Specific Aims: 1. Provide NMR instrumentation and expertise for characterizing macromolecules and their interactions;2. Provide the instrumentation and expertise for metabolomics studies;3. Facilitate structure-based drug design for drug discovery and drug development;4. Develop and implement new experiments and capabilities including stable isotope resolved metabolomics on small animals, in vivo;5. Educate members of the community on the specific capabilities of NMR for their research projects;6. Provide training of students and fellows in cancer biochemistry and drug development.
These Aims complement those of the other Cores in terms of the overall research mission of the Cancer Center and the University.

Public Health Relevance

NMR provides high resolution information about drug targets and their interactions, as well as metabolomics analyses at the atomic level that are critical to basic understanding of cellular metabolism and drug discovery and development.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Center Core Grants (P30)
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Special Emphasis Panel (ZGM1)
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University of Louisville
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Bardi, Gina T; Smith, Mary Ann; Hood, Joshua L (2018) Melanoma exosomes promote mixed M1 and M2 macrophage polarization. Cytokine 105:63-72
Neely, Aaron M; Zhao, Guoping; Schwarzer, Christian et al. (2018) N-(3-Oxo-acyl)-homoserine lactone induces apoptosis primarily through a mitochondrial pathway in fibroblasts. Cell Microbiol 20:
Ikeya, Teppei; Ban, David; Lee, Donghan et al. (2018) Solution NMR views of dynamical ordering of biomacromolecules. Biochim Biophys Acta Gen Subj 1862:287-306
Sabo, T Michael; Gapsys, Vytautas; Walter, Korvin F A et al. (2018) Utilizing dipole-dipole cross-correlated relaxation for the measurement of angles between pairs of opposing C?H?-C?H? bonds in anti-parallel ?-sheets. Methods 138-139:85-92
Hao, Jiaqing; Zhang, Yuwen; Yan, Xiaofang et al. (2018) Circulating Adipose Fatty Acid Binding Protein Is a New Link Underlying Obesity-Associated Breast/Mammary Tumor Development. Cell Metab 28:689-705.e5
Monsen, Robert C; Trent, John O (2018) G-quadruplex virtual drug screening: A review. Biochimie 152:134-148
Jones, Dominique Z; Schmidt, M Lee; Suman, Suman et al. (2018) Micro-RNA-186-5p inhibition attenuates proliferation, anchorage independent growth and invasion in metastatic prostate cancer cells. BMC Cancer 18:421
Woo, Sangsoon; Gao, Hong; Henderson, David et al. (2017) AKR1C1 as a Biomarker for Differentiating the Biological Effects of Combustible from Non-Combustible Tobacco Products. Genes (Basel) 8:
Shukla, Devesh; Rinehart, Claire A; Sahi, Shivendra V (2017) Comprehensive study of excess phosphate response reveals ethylene mediated signaling that negatively regulates plant growth and development. Sci Rep 7:3074
Al-Eryani, Laila; Waigel, Sabine; Jala, Venkatakrishna et al. (2017) Cell cycle pathway dysregulation in human keratinocytes during chronic exposure to low arsenite. Toxicol Appl Pharmacol 331:130-134

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