The mission ofthe Protein Structure Laboratory (PSL) is to provide investigators with state-of-the-art instrumentation, facilities, and expertise for all aspects of protein crystallography. This includes protein crystallization. X-ray data collection, data analysis, structure solution and refinement, and structure analysis. Laboratory staff provides advice and consultation, training, access to facilities for experienced crystallographers, and a range of services from crystal growth to structure solution and refinement, and preparation of materials for publications and grant submissions. In addition, the PSL provides training to students, post-docs and faculty who wish to learn any or all aspects of protein structure determination. To facilitate this, modern crystallization methods, in-house or synchrotron X-ray diffraction data collection, and the most recent crystallographic software are utilized. The staff consists of one experienced full time Ph.D. crystallographer. Dr. Scott Lovell, who serves as the Director ofthe Laboratory, and a Research Assistant to assist with crystallization screening and sample preparation for X-ray diffraction experiments. The PSL is located in close proximity to other core service laboratories in the University of Kansas Structural Biology Center building. During Phase II, the PSL has collaborated with 36 investigators from academic, government and industrial institutions. This has resulted in more than 100 structures delivered, 64 deposited to the Protein Databank, 26 publications and 17 grant applications submitted in which the PSL staff were listed. The PSL will continue to expand the base of collaborators and provide high-level facilities, expertise and training during Phase III ofthe COBRE-PSF.

Public Health Relevance

The Protein Structure Core Laboratory uses X-ray crystallography to determine detailed 3-dimensional structures of proteins for investigators. It also provides training in all relevant methods used in protein crystallography.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZGM1-TWD-C (C3))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Kansas Lawrence
United States
Zip Code
Arnett, David C; Bailey, Sheila K; Johnson, Carey K (2018) Exploring the conformations of nitric oxide synthase with fluorescence. Front Biosci (Landmark Ed) 23:2133-2145
Galasiti Kankanamalage, Anushka C; Kim, Yunjeong; Damalanka, Vishnu C et al. (2018) Structure-guided design of potent and permeable inhibitors of MERS coronavirus 3CL protease that utilize a piperidine moiety as a novel design element. Eur J Med Chem 150:334-346
Lan, Lan; Liu, Hao; Smith, Amber R et al. (2018) Natural product derivative Gossypolone inhibits Musashi family of RNA-binding proteins. BMC Cancer 18:809
Wu, Long; Zhang, Xin; Zhao, Liqin (2018) Human ApoE Isoforms Differentially Modulate Brain Glucose and Ketone Body Metabolism: Implications for Alzheimer's Disease Risk Reduction and Early Intervention. J Neurosci 38:6665-6681
Haimov, Ora; Sehrawat, Urmila; Tamarkin-Ben Harush, Ana et al. (2018) Dynamic interactions of eIF4G1 with eIF4E and eIF1 underlie scanning dependent and independent translation. Mol Cell Biol :
Herrera, Alvaro I; Ploscariu, Nicoleta T; Geisbrecht, Brian V et al. (2018) 1H, 15N, and 13C resonance assignments of the third domain from the S. aureus innate immune evasion protein Eap. Biomol NMR Assign 12:175-178
Murakami, Ryo; Singh, Chingakham Ranjit; Morris, Jacob et al. (2018) The Interaction between the Ribosomal Stalk Proteins and Translation Initiation Factor 5B Promotes Translation Initiation. Mol Cell Biol 38:
McGill, Jodi L; Kelly, Sean M; Kumar, Pankaj et al. (2018) Efficacy of mucosal polyanhydride nanovaccine against respiratory syncytial virus infection in the neonatal calf. Sci Rep 8:3021
Vasquez-Montes, Victor; Gerhart, Janessa; King, Kelly E et al. (2018) Comparison of lipid-dependent bilayer insertion of pHLIP and its P20G variant. Biochim Biophys Acta Biomembr 1860:534-543
Garabedian, Alyssa; Baird, Matthew A; Porter, Jacob et al. (2018) Linear and Differential Ion Mobility Separations of Middle-Down Proteoforms. Anal Chem 90:2918-2925

Showing the most recent 10 out of 68 publications