In this Phase III COBRE we will continue to develop the thematic focus: Smooth Muscle Plasticity, by continuing and enhancing Core laboratory services developed during COBRE Phases l&ll. Smooth muscles are complex tissues containing multiple cell types. Treating these muscles as homogenous tissues has been a common way to evaluate gene expression and the changes that occur in gene expression in conditions such as development and aging, diabetes, remodeling, responses to inflammation, etc. The smooth muscle biology group at the University of Nevada has pioneered techniques to sort the cellular components of smooth muscle tissues using cell-specific reporters and surface antigens and then to perform genome wide evaluations of cell-specific transcriptomes. Highly prominent genes in specific cell types are being evaluated with the Fluidigm Biomark HD technology to determine cell-by-cell quantitative changes in gene expression as smooth muscle tissues are altered in development or disease states. Transciptional analysis is also accompanied by evaluation of which transcripts are actively translated using Ribosome profiling techniques. We are also creating cell lines in which specific genes are up or down regulated. Services to the thematic focus of the COBRE will be provided by Core B (FACS/flow cytometry Shared Resource Lab) and Core C (Single Cell Molecular Expression). Detailed plans are provided to enhance the user base for these Cores throughout the University and to create long-lasting sustainable Core facilities for the research community in the State. The Phase III will be supplement by a Pilot Grant Program that is designed to increase usage of the Cores by several more projects within the University. Pilot Grants will be awarded to qualified investigators to obtain pilot data using Core technologies such that utilization ofthe Cores will enhance the strength of grant proposals and increase Core usage when new grants are funded. The Phase III is lead by an Administrative Core (Core A) which provides leadership via the Program Director and Program Coordinator, quality control via an Internal Steering Committee, and annual evaluation through an Assessment Coordinator and an External Advisory Committee.

Public Health Relevance

This center of biomedical research excellence (COBRE) is investigating the cells that generate contractile behaviors in smooth muscle tissues. We are isolated cellular components and studying how gene expression changes in development and disease states. The COBRE provides Core laboratories and pilot projects to allow cutting-edge technologies to be applied to smooth muscle research projects at UNR.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Nevada Reno
United States
Zip Code
Wang, Zhuqing; Lee, Sandy; Oliver, Daniel et al. (2018) Prps1l1, a testis-specific gene, is dispensable for mouse spermatogenesis. Mol Reprod Dev 85:802-804
Shi, Junchao; Ko, Eun-A; Sanders, Kenton M et al. (2018) SPORTS1.0: A Tool for Annotating and Profiling Non-coding RNAs Optimized for rRNA- and tRNA-derived Small RNAs. Genomics Proteomics Bioinformatics 16:144-151
Blanco, Luz P; Payne, Bryan L; Feyertag, Felix et al. (2018) Proteins of generalist and specialist pathogens differ in their amino acid composition. Life Sci Alliance 1:e201800017
Ben Maamar, Millissia; Sadler-Riggleman, Ingrid; Beck, Daniel et al. (2018) Alterations in sperm DNA methylation, non-coding RNA expression, and histone retention mediate vinclozolin-induced epigenetic transgenerational inheritance of disease. Environ Epigenet 4:dvy010
Zhang, Yunfang; Zhang, Xudong; Shi, Junchao et al. (2018) Dnmt2 mediates intergenerational transmission of paternally acquired metabolic disorders through sperm small non-coding RNAs. Nat Cell Biol 20:535-540
Tang, Chong; Klukovich, Rachel; Peng, Hongying et al. (2018) ALKBH5-dependent m6A demethylation controls splicing and stability of long 3'-UTR mRNAs in male germ cells. Proc Natl Acad Sci U S A 115:E325-E333
Skinner, Michael K; Ben Maamar, Millissia; Sadler-Riggleman, Ingrid et al. (2018) Alterations in sperm DNA methylation, non-coding RNA and histone retention associate with DDT-induced epigenetic transgenerational inheritance of disease. Epigenetics Chromatin 11:8
Singh, Mahendra; Miura, Pedro; Renden, Robert (2018) Age-related defects in short-term plasticity are reversed by acetyl-L-carnitine at the mouse calyx of Held. Neurobiol Aging 67:108-119
Baker, Salah A; Drumm, Bernard T; Skowronek, Karolina E et al. (2018) Excitatory Neuronal Responses of Ca2+ Transients in Interstitial Cells of Cajal in the Small Intestine. eNeuro 5:
Heredia, Dante J; Feng, Cheng-Yuan; Agarwal, Andrea et al. (2018) Postnatal Restriction of Activity-Induced Ca2+ Responses to Schwann Cells at the Neuromuscular Junction Are Caused by the Proximo-Distal Loss of Axonal Synaptic Vesicles during Development. J Neurosci 38:8650-8665

Showing the most recent 10 out of 42 publications