The goal of the UNMC DNA Microarray and Sequencing Core facility is to provide state-of-the art genetic research services to the regional academic research community. The core will contribute to the ongoing mission of the Molecular Biology of Neurosensory Systems COBRE by continuing to provide the functional genomics infrastructure necessary for COBRE researchers to conduct their research projects. DNA microarrays and next generation DNA sequencing are essential research tools that enable researchers to identify underlying biological mechanisms contributing to both normal and disease related development and physiology. They are critical components that help to facilitate the ultimate research goals of developing new treatment modalities to help patients with biomedical problems.These technologies require specialized equipment and technological expertise and as such are best performed in a core setting where the focus is specifically to develop assays and perform experiments for other researchers accessing the services. The core offers a variety of services to researchers including DNA Microarray as well as traditional and next generation DNA sequencing. In order to maximize the benefit of state-of-the art genomics technologies, it is imperative that a continual interaction between the core director, staff, and researchers is ongoing and the core is committed to this. In addition, genomics technology is undergoing rapid growth in the area of research applications, therefore the core will continually develop and implement new technologies as needed and work to obtain and/or upgrade necessary instrumentation. To ensure long-term sustainability, the vision for the core is to ensure that it continues to provide the services in a cost-efficient and timely fashion. The overall mission of this core is to provide essential services to researchers, communicate and train investigators in the use of these technologies, and stay current with respect to instrumentation and technological procedures, in order to help facilitate the long term goal of benefiting the health of patients.
In order to perform genetic research it is necessary to access highly technical and cost effective research services. The UNMC DNA Sequencing and Microarray Cores exist to provide these research services to the COBRE investigators as well as other members of the regional academic research community in the state of Nebraska.
|Leiferman, Amy; Shu, Jiang; Grove, Ryan et al. (2018) A diet defined by its content of bovine milk exosomes and their RNA cargos has moderate effects on gene expression, amino acid profiles and grip strength in skeletal muscle in C57BL/6 mice. J Nutr Biochem 59:123-128
|Miura, Hiromi; Quadros, Rolen M; Gurumurthy, Channabasavaiah B et al. (2018) Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors. Nat Protoc 13:195-215
|Won, Harim I; Schulze, Thomas T; Clement, Emalie J et al. (2018) De novo Assembly of the Burying Beetle Nicrophorus orbicollis (Coleoptera: Silphidae) Transcriptome Across Developmental Stages with Identification of Key Immune Transcripts. J Genomics 6:41-52
|Xia, Xiaohuan; Teotia, Pooja; Ahmad, Iqbal (2018) Lin28a regulates neurogliogenesis in mammalian retina through the Igf signaling. Dev Biol 440:113-128
|Baranovskiy, Andrey G; Duong, Vincent N; Babayeva, Nigar D et al. (2018) Activity and fidelity of human DNA polymerase ? depend on primer structure. J Biol Chem 293:6824-6843
|Booth, Kevin T; Askew, James W; Talebizadeh, Zohreh et al. (2018) Splice-altering variant in COL11A1 as a cause of nonsyndromic hearing loss DFNA37. Genet Med :
|Barta, Cody L; Liu, Huizhan; Chen, Lei et al. (2018) RNA-seq transcriptomic analysis of adult zebrafish inner ear hair cells. Sci Data 5:180005
|Alsaad, Hassan A; DeKorver, Nicholas W; Mao, Zhihao et al. (2018) In the Telencephalon, GluN2C NMDA Receptor Subunit mRNA is Predominately Expressed in Glial Cells and GluN2D mRNA in Interneurons. Neurochem Res :
|Cruz, Eric; Kumar, Sushil; Yuan, Li et al. (2018) Intracellular amyloid beta expression leads to dysregulation of the mitogen-activated protein kinase and bone morphogenetic protein-2 signaling axis. PLoS One 13:e0191696
|Liu, Huizhan; Chen, Lei; Giffen, Kimberlee P et al. (2018) Cell-Specific Transcriptome Analysis Shows That Adult Pillar and Deiters' Cells Express Genes Encoding Machinery for Specializations of Cochlear Hair Cells. Front Mol Neurosci 11:356
Showing the most recent 10 out of 72 publications