Basic Science Core II (BSCII) focuses on in vivo and translational aspects of basic neuroscience and encompasses animal models and their behavioral testing, histology, microscopy, neuropathology, and neuronal functional studies. The BSCII assists investigators with animal models and behavioral testing allowing them to overcome the challenges of developing and characterizing new rodent models, particularly with behavioral phenotypes, which may be outside their areas of expertise. The core offers several models of neuroAIDS, including immunodeficient mouse models of HIV encephalopathy, in vivo HIV-1 infection using humanized mice, transgenic models including the HIV Tg26 transgenic mouse and the GFAP-Tat Tet-inducible transgenic mouse, inducible and tissue specific transgenic, knockdown, or knockout models, cell transplantation models, viral vector delivery, etc. Expertise in behavioral testing for cognitive changes in learning and memory, as well as peripheral neuropathies are provided. Expertise in the core includes experience in breeding and phenotyping of neurological models, and neurobehavioral testing which can be particularly challenging for investigators without prior experience. Assistance is also provided with histological and immunohistochemical evaluation of human autopsy and biopsy clinical samples from patients with neurological disorders, including AIDs, PML, neurodegenerative diseases, and CNS neoplasia, tissues harvested from experimental animal models, cell cultures and access to neuropathological consultation. The core provides study design and data interpretation, and microscopy services, including Laser Capture Microdissection (LCM) accessioning of tissue samples and nucleic acid extraction for downstream molecular biological applications, quantitative microscopy and imaging, and data analysis. We also offer state-of-the-art microelectrode array (MEA) studies for neuronal function in cell culture and whole animal systems. A key component of the core is to provide the necessary training to the investigators and their staff to gain the proficiency needed to independently conduct any of the routine core functions. These services assist investigators in the neuroAIDS community with an array of histopathological and in vivo translational resources.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Center Core Grants (P30)
Project #
5P30MH092177-10
Application #
9931311
Study Section
Special Emphasis Panel (ZMH1)
Project Start
Project End
Budget Start
2020-06-01
Budget End
2021-05-31
Support Year
10
Fiscal Year
2020
Total Cost
Indirect Cost
Name
Temple University
Department
Type
DUNS #
057123192
City
Philadelphia
State
PA
Country
United States
Zip Code
19122
Mohseni Ahooyi, Taha; Shekarabi, Masoud; Decoppet, Emilie A et al. (2018) Network analysis of hippocampal neurons by microelectrode array in the presence of HIV-1 Tat and cocaine. J Cell Physiol 233:9299-9311
Tahrir, Farzaneh G; Shanmughapriya, Santhanam; Ahooyi, Taha Mohseni et al. (2018) Dysregulation of mitochondrial bioenergetics and quality control by HIV-1 Tat in cardiomyocytes. J Cell Physiol 233:748-758
Donadoni, Martina; Sariyer, Rahsan; Wollebo, Hassen et al. (2018) Viral tumor antigen expression is no longer required in radiation-resistant subpopulation of JCV induced mouse medulloblastoma cells. Genes Cancer 9:130-141
Cotto, Bianca; Natarajaseenivasan, Kalimuthusamy; Ferrero, Kimberly et al. (2018) Cocaine and HIV-1 Tat disrupt cholesterol homeostasis in astrocytes: Implications for HIV-associated neurocognitive disorders in cocaine user patients. Glia 66:889-902
Bella, Ramona; Kaminski, Rafal; Mancuso, Pietro et al. (2018) Removal of HIV DNA by CRISPR from Patient Blood Engrafts in Humanized Mice. Mol Ther Nucleic Acids 12:275-282
Mohseni Ahooyi, Taha; Shekarabi, Masoud; Torkzaban, Bahareh et al. (2018) Dysregulation of Neuronal Cholesterol Homeostasis upon Exposure to HIV-1 Tat and Cocaine Revealed by RNA-Sequencing. Sci Rep 8:16300
Craigie, Michael; Cicalese, Stephanie; Sariyer, Ilker Kudret (2018) Neuroimmune Regulation of JC Virus by Intracellular and Extracellular Agnoprotein. J Neuroimmune Pharmacol 13:126-142
Mele, Anthony R; Marino, Jamie; Chen, Kenneth et al. (2018) Defining the molecular mechanisms of HIV-1 Tat secretion: PtdIns(4,5)P2 at the epicenter. Traffic :
Delcour, Maxime; Russier, Michaƫl; Castets, Francis et al. (2018) Early movement restriction leads to maladaptive plasticity in the sensorimotor cortex and to movement disorders. Sci Rep 8:16328
Cotto, Bianca; Li, Hongbo; Tuma, Ronald F et al. (2018) Cocaine-mediated activation of microglia and microglial MeCP2 and BDNF production. Neurobiol Dis 117:28-41

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