The purpose of ?UNC Neuroscience Center Research Cores? (P30 NS045892-14) is to provide NINDS-funded and other NINDS-priority investigators with research capabilities that cannot be supported or sustained by individual laboratories. For the past 13 years, the Cores funded by NS045892 supported almost all NINDS- funded research at the UNC?Chapel Hill School of Medicine. During this time, UNC neuroscientists utilized these Cores to facilitate breakthrough discoveries in NINDS priority areas of neurogenetics, pain, neurodegeneration, and systems neuroscience. For our competitive renewal, we propose two Cores: Microscopy and Bioinformatics. We regularly upgraded our Microscopy Core to take advantage of increased sensitivity, scanning speeds, tiling capabilities, and now super-resolution capabilities. In addition to a Zeiss LSM 780 and other scopes, our Microscopy Core will house a new Zeiss LSM 880 with Airyscan and Fast module. The Core will also provide IT infrastructure and a pipeline for computationally intensive image processing and analysis of light sheet microscopy data. For the Bioinformatics Core, we will fund two bioinformaticians to analyze high-throughput sequencing (HTS) data, including exome and genome sequencing, bulk and single-cell RNA-seq, bulk and single-cell ATAC-seq, Hi-C, and ChIP-seq data. Access to advanced microscopic imaging and bioinformatics support is essential to the performance of cutting- edge research in NINDS-priority areas. With continued support, our NINDS-funded and other NINDS-priority investigators will have their transformative ideas enabled via convenient and affordable access to these key technologies.
?UNC Neuroscience Center Research Cores? (P30 NS045892-14) supports almost all NINDS-funded research at the UNC?Chapel Hill School of Medicine. We request continued support for Cores that provide cutting edge resources in Microscopy and Bioinformatics. These Cores provide the tools that our NINDS-funded investigators need to conduct research directed at curing neurological diseases.
|Song, Liujiang; Llanga, Telmo; Conatser, Laura M et al. (2018) Serotype survey of AAV gene delivery via subconjunctival injection in mice. Gene Ther 25:402-414|
|Zhang, Jing; Wu, Tao; Simon, Jeremy et al. (2018) VHL substrate transcription factor ZHX2 as an oncogenic driver in clear cell renal cell carcinoma. Science 361:290-295|
|Boyer, Nicholas P; Monkiewicz, Caroline; Menon, Shalini et al. (2018) Mammalian TRIM67 Functions in Brain Development and Behavior. eNeuro 5:|
|Sidorov, Michael S; Judson, Matthew C; Kim, Hyojin et al. (2018) Enhanced Operant Extinction and Prefrontal Excitability in a Mouse Model of Angelman Syndrome. J Neurosci 38:2671-2682|
|Crowther, Andrew J; Lim, Szu-Aun; Asrican, Brent et al. (2018) An Adeno-Associated Virus-Based Toolkit for Preferential Targeting and Manipulating Quiescent Neural Stem Cells in the Adult Hippocampus. Stem Cell Reports 10:1146-1159|
|Allard, Denise E; Wang, Yan; Li, Jian Joel et al. (2018) Schwann cell-derived periostin promotes autoimmune peripheral polyneuropathy via macrophage recruitment. J Clin Invest 128:4727-4741|
|Yeh, Chia-Yu; Asrican, Brent; Moss, Jonathan et al. (2018) Mossy Cells Control Adult Neural Stem Cell Quiescence and Maintenance through a Dynamic Balance between Direct and Indirect Pathways. Neuron 99:493-510.e4|
|Thaxton, Courtney; Kloth, Alexander D; Clark, Ellen P et al. (2018) Common Pathophysiology in Multiple Mouse Models of Pitt-Hopkins Syndrome. J Neurosci 38:918-936|
|Decot, Heather K; Namboodiri, Vijay M K; Gao, Wei et al. (2017) Coordination of Brain-Wide Activity Dynamics by Dopaminergic Neurons. Neuropsychopharmacology 42:615-627|
|McCoy, Eric S; Taylor-Blake, Bonnie; Aita, Megumi et al. (2017) Enhanced Nociception in Angelman Syndrome Model Mice. J Neurosci 37:10230-10239|
Showing the most recent 10 out of 158 publications