We are requesting NINDS funding for continued support of the NeuroProteomics Core Facility within the Center for Advanced Proteomics Research (CAPR) at the University of Medicine and Dentistry of New Jersey (UMDNJ). During the past funding period, this Core has successfully advanced both neuroscience research and educational missions. It has provided NINDS researchers access to advanced proteomics technologies, and has served as a hub for collaborations and new technology developments. In this renewal application, we are proposing to provide NINDS-funded Pi's with access to the latest state-of-the- art technologies with upgraded Core equipments. These expanded capabilities include an Applied Biosystems 4800 tandem mass spectrometer and other latest proteomics equipments for high throughput protein biomarker discovery and validation. Critical functions that will be fulfilled by these technology upgrades include: 1) more sensitive identification and 2) quantification of protein phosphorylation sites and other post-translational modifications, these systems will significantly enhance the productivity of the eleven qualifying and other neuroscience research projects (including over 30 NINDS investigators). Successful application of proteomics requires both advanced instrumentation and experienced operators. A NINDS core grant will provide dedicated personnel proficient in sample preparation, 2D gel electrophoresis, mass spectrometry and bioinformatics, as well as instrument time and supplies associated with NINDS qualifying projects, a resource that will not overlap with existing facilities. To ensure the continuous success of the Core, UMDNJ has provided excellent institutional commitments towards this Core including new laboratory space, instrumentation upgrade and salary support for the supporting personnel. Two committees, composed of both NINDS and other CAPR investigators, will oversee the Core's fiscal and scientific management. A User Club will provide an important forum for education, training, data sharing and collaborations. As demonstrated by the Core's past record of success and its sound scientific and financial management structure, this improved Core will be well utilized to enhance research capabilities for NINDS-sponsored studies of human diseases including multiple sclerosis, Parkinson's and other neurodegenerative diseases. With the benefit of a NINDS grant, neuroscience investigators will use proteomics technology to maximum benefits, to complement their existing research approaches and promote new research directions and collaborations.
We are requesting NINDS funding for the continued support of the NeuroProteomics Core Facility at the University of Medicine and Dentistry of New Jersey. This facility will improve our researchers'ability to study protein abnormalities in neurodegeneration, aging and other diseases.
|Wu, Changgong; Liu, Tong; Wang, Yan et al. (2018) Biotin Switch Processing and Mass Spectrometry Analysis of S-Nitrosated Thioredoxin and Its Transnitrosation Targets. Methods Mol Biol 1747:253-266|
|Su, Wen-Min; Han, Gil-Soo; Dey, Prabuddha et al. (2018) Protein kinase A phosphorylates the Nem1-Spo7 protein phosphatase complex that regulates the phosphorylation state of the phosphatidate phosphatase Pah1 in yeast. J Biol Chem 293:15801-15814|
|Solé-Domènech, Santiago; Rojas, Ana V; Maisuradze, Gia G et al. (2018) Lysosomal enzyme tripeptidyl peptidase 1 destabilizes fibrillar A? by multiple endoproteolytic cleavages within the ?-sheet domain. Proc Natl Acad Sci U S A 115:1493-1498|
|Zhong, Fang; Chen, Haibing; Xie, Yifan et al. (2018) Protein S Protects against Podocyte Injury in Diabetic Nephropathy. J Am Soc Nephrol 29:1397-1410|
|Yan, Run; Zhang, Jie; Park, Hye-Jin et al. (2018) Synergistic neuroprotection by coffee components eicosanoyl-5-hydroxytryptamide and caffeine in models of Parkinson's disease and DLB. Proc Natl Acad Sci U S A 115:E12053-E12062|
|Heffernan, Corey; Jain, Mohit R; Liu, Tong et al. (2017) Nectin-like 4 Complexes with Choline Transporter-like Protein-1 and Regulates Schwann Cell Choline Homeostasis and Lipid Biogenesis in Vitro. J Biol Chem 292:4484-4498|
|Sleat, David E; Tannous, Abla; Sohar, Istvan et al. (2017) Proteomic Analysis of Brain and Cerebrospinal Fluid from the Three Major Forms of Neuronal Ceroid Lipofuscinosis Reveals Potential Biomarkers. J Proteome Res 16:3787-3804|
|Zhou, Junsong; Wu, Yi; Chen, Fengwu et al. (2017) The disulfide isomerase ERp72 supports arterial thrombosis in mice. Blood 130:817-828|
|Geng, Ke; Kumar, Sushil; Kimani, Stanley G et al. (2017) Requirement of Gamma-Carboxyglutamic Acid Modification and Phosphatidylserine Binding for the Activation of Tyro3, Axl, and Mertk Receptors by Growth Arrest-Specific 6. Front Immunol 8:1521|
|Wu, Changgong; Dai, Huacheng; Yan, Lin et al. (2017) Sulfonation of the resolving cysteine in human peroxiredoxin 1: A comprehensive analysis by mass spectrometry. Free Radic Biol Med 108:785-792|
Showing the most recent 10 out of 81 publications