The development of advanced microscopy techniques is a driving force behind advances in biological research and medical imaging. The refinement and commercialization of laser scanning confocal microscopes, nanoscopes and other types of imaging instruments along with advances in fluorophore development have led to a revolution in biological microscopy (Buckers et al., 2011;Wang et al.,2011;Reck-Peterson et al., 2010). The newest imaging methods, however, often require expertise and instruments beyond the financial and technical capacities of most individual scientists. Thus, shared imaging facilities are crucial components of university research cores. The ICAC is the only shared imaging facility within Tufts Medical School and Tufts Medical center. It was developed in response to the rapid ad vances occurring in contemporary biological microscopy and to meet the imaging priorities of Tufts neuroscience investigators. The ICAC has provided Tufts neuroscientists with a broad range of microscopic imaging services including wide-field fluorescence microscopy &digital imaging, confocal microscopy, 2-photon microscopy, Total Internal Reflection Fluorescence (TIRF) microscopy, electron microscopy (EM), live-cell imaging, and laser capture microdissection. Equipment is available for standard microscopy as well as dynamic imaging such as fluorescence resonance energy transfer (FRET) or fluorescence recovery after photobleaching (FRAP) experiments. The ICAC has been heavily used since its inception;it was used by 39-46 labs and 62-70 individuals during every year of this funding cycle. Thirty one neuroscience labs including 9 NINDS-funded labs employed ICAC facilities during this cycle. Given the heavy core usage, we recently added a second confocal microscope and a TIRF microscope to the facility using supplemental funds from NINDS and a shared instrumentation grant from NCRR (see Section B5

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National Institute of Neurological Disorders and Stroke (NINDS)
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You, Samantha; Fulga, Tudor A; Van Vactor, David et al. (2018) Regulation of Circadian Behavior by Astroglial MicroRNAs in Drosophila. Genetics 208:1195-1207
Ahumada-Castro, Ulises; Silva-Pavez, Eduardo; Lovy, Alenka et al. (2018) MTOR-independent autophagy induced by interrupted endoplasmic reticulum-mitochondrial Ca2+ communication: a dead end in cancer cells. Autophagy :1-4
Dickson, David A; Paulus, Jessica K; Mensah, Virginia et al. (2018) Reduced levels of miRNAs 449 and 34 in sperm of mice and men exposed to early life stress. Transl Psychiatry 8:101
Xu, Jie; Bartolome, Christopher L; Low, Cho Shing et al. (2018) Genetic identification of leptin neural circuits in energy and glucose homeostases. Nature 556:505-509
Chen, Zhigang; Li, Wenlu; Qiu, Fuming et al. (2018) Aspirin cooperates with p300 to activate the acetylation of H3K9 and promote FasL-mediated apoptosis of cancer stem-like cells in colorectal cancer. Theranostics 8:4447-4461
Melón, Laverne Camille; Hooper, Andrew; Yang, Xuzhong et al. (2018) Inability to suppress the stress-induced activation of the HPA axis during the peripartum period engenders deficits in postpartum behaviors in mice. Psychoneuroendocrinology 90:182-193
Kim, WonHee; Ma, Liang; Lomoio, Selene et al. (2018) BACE1 elevation engendered by GGA3 deletion increases ?-amyloid pathology in association with APP elevation and decreased CHL1 processing in 5XFAD mice. Mol Neurodegener 13:6
Cai, Weikang; Xue, Chang; Sakaguchi, Masaji et al. (2018) Insulin regulates astrocyte gliotransmission and modulates behavior. J Clin Invest 128:2914-2926
Shaban, Lamyaa; Chen, Ying; Fasciano, Alyssa C et al. (2018) A 3D intestinal tissue model supports Clostridioides difficile germination, colonization, toxin production and epithelial damage. Anaerobe 50:85-92
Hooper, Andrew; Paracha, Rumzah; Maguire, Jamie (2018) Seizure-induced activation of the HPA axis increases seizure frequency and comorbid depression-like behaviors. Epilepsy Behav 78:124-133

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