It is often averred in the Life Sciences that we are not short of questions; rather, we are limited by the availability of technologies to address these questions. During the past decade several new technologies have been developed to address biological problems. However, barriers to using these newly-developed technologies are often considerable for the individual investigator. The goal of this Center is to provide a centralized and staffed facility to allow both the expert and the novice to use the most modern research tools in their studies, and to equip these instruments for dynamic imaging in physiological studies. We will develop a Center around three cores Dynamic Imaging Core, containing, two two-photon microscopes, spectral detection confocal microscopes, total internal reflectance fluorescence microscope together with photolysis attachments; Data Analysis Core that will provide opportunities for off-line analysis of all data collected with the Center's microscopes, and Consultation Core for new and established users of the Center's Dynamic Imaging Core. The goals of this Center fall into three scientific clusters: I) Neuronal Development, in which ot projects will investigate problems ranging from stem cell biology to neuronal migration and synapse formation, lI) Regulation of Ion Channels and Synaptie Transmission, in which our studies include protein-protein interactions controlling calcium channel activity, the spatial distribution of receptors, and the localization of synapses controlling neuronal networks, and Ill) Gila-Neuron Interactions, in which we will investigate calcium signaling in astrocytes, the roles of ion channels in myelination, and the role of glial-released messengers in synapse formation. Establishing this Dynamic Imaging Center will catalyze new studies and augment existing studies in the 21 qualifying N1NDS-funded laboratories (including 34 N1NDS grants) that comprise this Center. The resultant synergistic interactions between faculty members will revolutionize our ability to study basic functions of the nervous system function and will ultimately enhance our understanding of these functions in human health and disease.
| Yu, Esther P; Dengler, Christopher G; Frausto, Shanti F et al. (2013) Protracted postnatal development of sparse, specific dentate granule cell activation in the mouse hippocampus. J Neurosci 33:2947-60 |
| Takano, Hajime; McCartney, Melissa; Ortinski, Pavel I et al. (2012) Deterministic and stochastic neuronal contributions to distinct synchronous CA3 network bursts. J Neurosci 32:4743-54 |
| Zhang, Junxian; Scherer, Steven S; Yum, Sabrina W (2011) Dominant Cx26 mutants associated with hearing loss have dominant-negative effects on wild type Cx26. Mol Cell Neurosci 47:71-8 |
| Yum, Sabrina W; Zhang, Junxian; Scherer, Steven S (2010) Dominant connexin26 mutants associated with human hearing loss have trans-dominant effects on connexin30. Neurobiol Dis 38:226-36 |
| D'Ascenzo, Marcello; Fellin, Tommaso; Terunuma, Miho et al. (2007) mGluR5 stimulates gliotransmission in the nucleus accumbens. Proc Natl Acad Sci U S A 104:1995-2000 |
| Halassa, Michael M; Fellin, Tommaso; Takano, Hajime et al. (2007) Synaptic islands defined by the territory of a single astrocyte. J Neurosci 27:6473-7 |
| Yum, Sabrina W; Zhang, Junxian; Valiunas, Virginijus et al. (2007) Human connexin26 and connexin30 form functional heteromeric and heterotypic channels. Am J Physiol Cell Physiol 293:C1032-48 |
| Pan, Zongming; Kao, Tingching; Horvath, Zsolt et al. (2006) A common ankyrin-G-based mechanism retains KCNQ and NaV channels at electrically active domains of the axon. J Neurosci 26:2599-613 |
| Barrett, Lindy E; Sul, Jai-Yoon; Takano, Hajime et al. (2006) Region-directed phototransfection reveals the functional significance of a dendritically synthesized transcription factor. Nat Methods 3:455-60 |
| Zhang, Guixin; Jin, Li-Qing; Sul, Jai-Yoon et al. (2005) Live imaging of regenerating lamprey spinal axons. Neurorehabil Neural Repair 19:46-57 |
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