EMBRYONIC STEM CELL CORE CI B1. Establishment of the ES Cell Core: The ES Cell Core was founded with the support of this award and began operations -four years ago. It is located on the 9th Floor of the Wood Basic Science Building in -500 sq. ft. of space that is contiguous with the JHU SOM Transgenic Animal Facility. Dr. Woriey serves as the Director of the ES Cell Core and works closely with Dr. Roger Reeves, Director of the Transgenic Animal Facility, to integrate the functions of these Cores (see letter of support from Dr. Reeves (consultant)). Ms. Holly Wellington serves as Core Manager for this facility. The ES Cell Core reviews the design of targeting constructs, electroporates targeting construct DNA into ES cells, and then selects and amplifies ES cell colonies in duplicate 96 well plates;one plate is cryogenically stored and the other provided to investigators to screen for homologous recombination. The Core then grows promising ES cell clones, checks karyotypes and provides the targeted ES cells to the Transgenic Facility for injection into blastocysts.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
5P30NS050274-09
Application #
8585922
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2013-12-01
Budget End
2014-11-30
Support Year
9
Fiscal Year
2014
Total Cost
$181,640
Indirect Cost
$70,884
Name
Johns Hopkins University
Department
Type
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218
Kajstura, Tymoteusz J; Dougherty, Sarah E; Linden, David J (2018) Serotonin axons in the neocortex of the adult female mouse regrow after traumatic brain injury. J Neurosci Res 96:512-526
Babola, Travis A; Li, Sally; Gribizis, Alexandra et al. (2018) Homeostatic Control of Spontaneous Activity in the Developing Auditory System. Neuron 99:511-524.e5
Dresselhaus, Erica C; Boersma, Matthew C H; Meffert, Mollie K (2018) Targeting of NF-?B to Dendritic Spines Is Required for Synaptic Signaling and Spine Development. J Neurosci 38:4093-4103
Larson, Valerie A; Mironova, Yevgeniya; Vanderpool, Kimberly G et al. (2018) Oligodendrocytes control potassium accumulation in white matter and seizure susceptibility. Elife 7:
Jiang, Zheng; Yue, Wendy W S; Chen, Lujing et al. (2018) Cyclic-Nucleotide- and HCN-Channel-Mediated Phototransduction in Intrinsically Photosensitive Retinal Ganglion Cells. Cell 175:652-664.e12
Hughes, Ethan G; Orthmann-Murphy, Jennifer L; Langseth, Abraham J et al. (2018) Myelin remodeling through experience-dependent oligodendrogenesis in the adult somatosensory cortex. Nat Neurosci 21:696-706
Minamisawa, Genki; Kwon, Sung Eun; Chevée, Maxime et al. (2018) A Non-canonical Feedback Circuit for Rapid Interactions between Somatosensory Cortices. Cell Rep 23:2718-2731.e6
Zhang, Ke; Daigle, J Gavin; Cunningham, Kathleen M et al. (2018) Stress Granule Assembly Disrupts Nucleocytoplasmic Transport. Cell 173:958-971.e17
Chevée, Maxime; Robertson, Johanna De Jong; Cannon, Gabrielle Heather et al. (2018) Variation in Activity State, Axonal Projection, and Position Define the Transcriptional Identity of Individual Neocortical Projection Neurons. Cell Rep 22:441-455
Langseth, Abraham J; Kim, Juhyun; Ugolino, Janet E et al. (2017) Cell-type specific differences in promoter activity of the ALS-linked C9orf72 mouse ortholog. Sci Rep 7:5685

Showing the most recent 10 out of 83 publications