A Molecular Analysis Core is proposed for providing basic and clinical researchers with consultation andexpertise in genomics and proteomics technology, experimental design, and data interpretation fortranslational studies in diseases of the nervous system. Facilitated access to the Affymetrix GeneChipmicroarray platform for performing gene expression analysis and array-based 'whole genome' SNPgenotyping, the Sequenom technology platform for performing targeted genotyping on large numbers ofsamples, and candidate gene capillary resequencing for mutational profiling studies will be provided.Neuroscience investigators will have preferred access to current and innovative proteomics technology.These will include instrumentation and methods for the separation of complex protein mixtures, robotics forsample processing, and protein identification and characterization (e.g. covalent modifications) using 'state-of-the-art' tandem mass spectrometry coupled to automated multi-dimensional capillary liquidchromatographs. Proposed neuroproteomic themes will be discovery and validation of protein biomarkers,measuring in vivo metabolic half- lives of disease-associated proteins in humans, defining functional proteinmodifications on receptors and ion channels, and characterizing novel protein complexes. Investigators willhave facilitated access to genomics and proteomics data both within Core C and through the coordinated,interdisciplinary activities of the Administrative Core A. Statistical evaluation, data management andintegration will be provided with the proposed Informatics Core G. The application of these new technologiesto diseases of the nervous system promises to revolutionize our understanding of these disorders and willhelp turn basic neurobiological discovery into diagnostic and therapeutic advances.
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