(GVVC) As one of three Stanford Neuroscience Cores, the Gene Vector and Virus Core (GVVC) provides viral vectors to manipulate and express recombinant proteins in the whole brain, in slices, and in cellular systems in a temporally and spatially controlled manner. The ability to employ these molecular techniques is essential to the daily research activities of a significant number of neuroscience labs at Stanford, and with the continued development of new molecular tools to report on and to manipulate neuronal function, and to trace neuronal connections with greater specificity, these techniques will remain critical to advancing our progress in understanding brain function. Over the past 4 years the GVVC has made 1348 viruses and provided 219 pre- made stock viruses for 581 projects. Moreover, GVVC has supported the viral production for the innovative optogenetics technology, in which light-sensitive channels (channelrhodopsins) are used to regulate neuronal activity, invented by the Dr. Karl Deisseroth laboratory. Logistical proximity of GVVC and its support for Deisseroth laboratory has led to dissemination of this technology not only to Stanford faculty but to many other neuroscientists nationwide. Since its inception, GVVC has continued to add to the number of stock viruses in its virus repository (http://med.stanford.edu/gvvc/stockviruses.html) which is accessible for distribution. Under the proposed grant, GVVC will continue to provide its present cutting-edge services. In addition, GVVC will develop new services, such as CAV-2 (canine adenovirus) virus, that will allow retrograde axonal transportation.
for Gene Vector and Virus Core (GVVC) Today our ability to study how the nervous system evolves during development, aging, learning, life experiences, or during disease progression is fundamentally dependent on our ability to make genetic constructs and viral products for genetic manipulation. As part of the Stanford Neuroscience Cores, the Gene Vector and Virus Core (GVVC) has provided viral vectors to manipulate and express recombinant proteins in the whole brain, in slices, and in cellular systems in a temporally and spatially controlled manner. These tools are essential for the research of a large part of the neuroscience community.
|Kramer, Nicholas J; Haney, Michael S; Morgens, David W et al. (2018) CRISPR-Cas9 screens in human cells and primary neurons identify modifiers of C9ORF72 dipeptide-repeat-protein toxicity. Nat Genet 50:603-612|
|Li, Shi-Bin; Nevárez, Natalie; Giardino, William J et al. (2018) Optical probing of orexin/hypocretin receptor antagonists. Sleep 41:|
|Liu, Qing; Van Bortle, Kevin; Zhang, Yue et al. (2018) Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid. Sci Rep 8:12960|
|Razavi, Mehdi; Thakor, Avnesh S (2018) An oxygen plasma treated poly(dimethylsiloxane) bioscaffold coated with polydopamine for stem cell therapy. J Mater Sci Mater Med 29:54|
|Giardino, William J; Eban-Rothschild, Ada; Christoffel, Daniel J et al. (2018) Parallel circuits from the bed nuclei of stria terminalis to the lateral hypothalamus drive opposing emotional states. Nat Neurosci 21:1084-1095|
|Bennett, F Chris; Bennett, Mariko L; Yaqoob, Fazeela et al. (2018) A Combination of Ontogeny and CNS Environment Establishes Microglial Identity. Neuron 98:1170-1183.e8|
|Wilson, Katheryne E; Bachawal, Sunitha V; Willmann, Jürgen K (2018) Intraoperative Resection Guidance with Photoacoustic and Fluorescence Molecular Imaging Using an Anti-B7-H3 Antibody-Indocyanine Green Dual Contrast Agent. Clin Cancer Res 24:3572-3582|
|Bui, Anh D; Nguyen, Theresa M; Limouse, Charles et al. (2018) Dentate gyrus mossy cells control spontaneous convulsive seizures and spatial memory. Science 359:787-790|
|Sun, Lu O; Mulinyawe, Sara B; Collins, Hannah Y et al. (2018) Spatiotemporal Control of CNS Myelination by Oligodendrocyte Programmed Cell Death through the TFEB-PUMA Axis. Cell 175:1811-1826.e21|
|Razavi, Mehdi; Hu, Sophia; Thakor, Avnesh S (2018) A collagen based cryogel bioscaffold coated with nanostructured polydopamine as a platform for mesenchymal stem cell therapy. J Biomed Mater Res A 106:2213-2228|
Showing the most recent 10 out of 102 publications