(BFNL) As one of the three Stanford Neuroscience Cores, the Stanford Behavioral and Functional Neuroscience Laboratory (BFNL). This core facility provides a centralized resource for standard and automated sensorimotor, learning and memory, and social behavioral paradigms as well as expertise in rodent models of CNS disorders in conjunction to pharmacological and experimental drug testing. In addition, automated behavior testing paradigms established by this core have helped our community to further address the standardization and reproducibility of behavioral tests using state-of-the-art apparatuses such as Intellicage and PhenoLab automated home environment monitoring systems. These systems provide complete home- cage sensorimotor behavioral monitoring capabilities and can test simple and complex learning and memory tasks in a fully automated fashion with minimal human intervention. Using this expertise and technology BFNL has provided state-of-the-art phenotyping services for novel transgenic lines and provided validated CNS Disease Models for testing novel compounds and neuropharmacological exploration during the past several years. BFNL has freely shared experimental protocols and data. The detailed list of behavioral paradigms, standard operating procedures, protocols, and a sample data set for most behavioral paradigm has been shared publicly at http://sbfnl.stanford.edu/testingtable. Over the past four years, BFNL has supported 55 labs and faculty with almost 40,000 hours of services. This effort has led to more than 20 publications overall. Going forward BFNL will continue providing these services and allocate additional resources to address more actively some of the rising concerns in the field about the predictive value and inherent limitations of animal models in drug discovery and also innovate and expand upon its automated testing capabilities. All of these advances will be shared with the neuroscience community via our newly improved resource sharing websites.

Public Health Relevance

(BFNL) The essential function of the Stanford Behavioral and Functional Neuroscience Laboratory (BFNL) is to provide a shared resource platform for academic labs, providing access to rodent behavior testing in existing and novel paradigms for characterizing behavior. This core facility provides a centralized resource for standard and automated sensorimotor, learning and memory, and social behavioral paradigms as well as expertise in rodent models of CNS disorders in conjunction to pharmacological and experimental drug testing.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
5P30NS069375-09
Application #
9593523
Study Section
Special Emphasis Panel (ZNS1)
Project Start
Project End
2020-11-30
Budget Start
2018-12-01
Budget End
2019-11-30
Support Year
9
Fiscal Year
2019
Total Cost
Indirect Cost
Name
Stanford University
Department
Type
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305
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Zhang, Zhenjie; Marro, Samuele G; Zhang, Yingsha et al. (2018) The fragile X mutation impairs homeostatic plasticity in human neurons by blocking synaptic retinoic acid signaling. Sci Transl Med 10:
Joshi, Amit U; Saw, Nay L; Shamloo, Mehrdad et al. (2018) Drp1/Fis1 interaction mediates mitochondrial dysfunction, bioenergetic failure and cognitive decline in Alzheimer's disease. Oncotarget 9:6128-6143
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Kramer, Nicholas J; Haney, Michael S; Morgens, David W et al. (2018) CRISPR-Cas9 screens in human cells and primary neurons identify modifiers of C9ORF72 dipeptide-repeat-protein toxicity. Nat Genet 50:603-612
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Liu, Qing; Van Bortle, Kevin; Zhang, Yue et al. (2018) Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid. Sci Rep 8:12960
Razavi, Mehdi; Thakor, Avnesh S (2018) An oxygen plasma treated poly(dimethylsiloxane) bioscaffold coated with polydopamine for stem cell therapy. J Mater Sci Mater Med 29:54
Giardino, William J; Eban-Rothschild, Ada; Christoffel, Daniel J et al. (2018) Parallel circuits from the bed nuclei of stria terminalis to the lateral hypothalamus drive opposing emotional states. Nat Neurosci 21:1084-1095
Bennett, F Chris; Bennett, Mariko L; Yaqoob, Fazeela et al. (2018) A Combination of Ontogeny and CNS Environment Establishes Microglial Identity. Neuron 98:1170-1183.e8

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