The major goals of this core were to establish a core facility for the high content analysis of neurons and neural networks.
The aim was started with high content cellular imaging systems from BD biosciences to capture fluorescence images of cells in 96 and 384 well format as necessary for morphology based shRNA and drug screens. Since then we have significantly expanded the imaging resources with the addition of an Olympus VS120 slide scanning microscope, a Leica laser capture microdissection system, an Olympus Viva View environmental time lapse imaging microscope, and a home built selective plane imaging microscope (SPIM). With these technologies the core has the capabilities to analyze cells and circuits at a wide range of spatiotemporal resolutions in order to reveal their anatomical organization as well as the relationship between cellular content and function. The major goals have not changed but the capabilities have been expanded via the acquisition of the components described above.
|Cheadle, Lucas; Tzeng, Christopher P; Kalish, Brian T et al. (2018) Visual Experience-Dependent Expression of Fn14 Is Required for Retinogeniculate Refinement. Neuron 99:525-539.e10|
|Hrvatin, Sinisa; Hochbaum, Daniel R; Nagy, M Aurel et al. (2018) Single-cell analysis of experience-dependent transcriptomic states in the mouse visual cortex. Nat Neurosci 21:120-129|
|Renthal, William; Boxer, Lisa D; Hrvatin, Sinisa et al. (2018) Characterization of human mosaic Rett syndrome brain tissue by single-nucleus RNA sequencing. Nat Neurosci 21:1670-1679|
|Sieker, Jakob T; Proffen, Benedikt L; Waller, Kimberly A et al. (2018) Transcriptional profiling of synovium in a porcine model of early post-traumatic osteoarthritis. J Orthop Res :|
|Wong, Man Yan; Liu, Changliang; Wang, Shan Shan H et al. (2018) Liprin-?3 controls vesicle docking and exocytosis at the active zone of hippocampal synapses. Proc Natl Acad Sci U S A 115:2234-2239|
|de Jong, Arthur P H; Roggero, Carlos M; Ho, Meng-Ru et al. (2018) RIM C2B Domains Target Presynaptic Active Zone Functions to PIP2-Containing Membranes. Neuron 98:335-349.e7|
|Liu, Changliang; Kershberg, Lauren; Wang, Jiexin et al. (2018) Dopamine Secretion Is Mediated by Sparse Active Zone-like Release Sites. Cell 172:706-718.e15|
|Martínez-François, Juan Ramón; Fernández-Agüera, María Carmen; Nathwani, Nidhi et al. (2018) BAD and KATP channels regulate neuron excitability and epileptiform activity. Elife 7:|
|Chantre, Christophe O; Campbell, Patrick H; Golecki, Holly M et al. (2018) Production-scale fibronectin nanofibers promote wound closure and tissue repair in a dermal mouse model. Biomaterials 166:96-108|
|Jeanne, James M; Fi?ek, Mehmet; Wilson, Rachel I (2018) The Organization of Projections from Olfactory Glomeruli onto Higher-Order Neurons. Neuron 98:1198-1213.e6|
Showing the most recent 10 out of 68 publications