We propose to continue the COBRE on Membrane Protein Production and Characterization at the University of Delaware as a Phase III COBRE infrastructure center. The critical role of membrane proteins in biology is clearly reflected in the fact that they represent >30% of the human genome and are the targets of >50% of all therapeutics on the market today. Nevertheless, significant challenges remain in producing and characterizing them, especially within the realm of structural biology. Consequently the objectives of research in the COBRE will be to express, solubilize, purify and crystallize membrane proteins;to determine their structures;and to characterize their functions at the molecular level and in larger biological systems in both health and disease. These objectives are congruent with those of the Structural Biology component of the NIH Roadmap and the Protein Structure Initiative in addressing an area of critical importance for biomedical research. The infrastructure center will complement research in the laboratories of an interdisciplinary group of more than a dozen Pis, whose work is independently supported. To this end the center will include: research and instrumentation cores (Protein Expression and Biophysical Characterization;Structural Biology;and a group of Core Facilities co-funded with, among others, the Delaware INBRE);continuation of the current pilot research subproject program;a predoctoral training program modeled on the NIH T32 mechanism;and continuation of the existing annual symposium series. The administration of the center will be guided by an internal Steering Committee and will continue to benefit from the input of an External Advisory Committee, including for review of pilot project proposals. The research within the COBRE coupled with the capabilities provided by the infrastructure center will continue to give the University of Delaware distinctive strength in an area of research critical to biology and biomedicine.
(provided by applicant): Membrane proteins exist in the membrane surrounding each cell and are critical to cellular growth, communication and nutrition, yet how these proteins function is still relatively poorly understood. The purpose of this center is to provide infrastructure for research to develop improved methods to produce and study membrane proteins, which can yield major benefits for basic biology and drug development.
|Naranjo, Andrea N; McNeely, Patrick M; Katsaras, John et al. (2016) Impact of purification conditions and history on A2A adenosine receptor activity: The role of CHAPS and lipids. Protein Expr Purif 124:62-7|
|Choi, Yong Seok; Lee, Kelvin H (2016) Multiple reaction monitoring assay based on conventional liquid chromatography and electrospray ionization for simultaneous monitoring of multiple cerebrospinal fluid biomarker candidates for Alzheimer's disease. Arch Pharm Res 39:390-7|
|Modla, Shannon; Caplan, Jeffrey L; Czymmek, Kirk J et al. (2015) Localization of fluorescently tagged protein to plasmodesmata by correlative light and electron microscopy. Methods Mol Biol 1217:121-33|
|Lee, Jung-Youn (2015) Plasmodesmata: a signaling hub at the cellular boundary. Curr Opin Plant Biol 27:133-40|
|Cui, Weier; Wang, Xu; Lee, Jung-Youn (2015) Drop-ANd-See: a simple, real-time, and noninvasive technique for assaying plasmodesmal permeability. Methods Mol Biol 1217:149-56|
|Min, Lie; Choe, Leila H; Lee, Kelvin H (2015) Improved protease digestion conditions for membrane protein detection. Electrophoresis 36:1690-8|
|Naranjo, Andrea N; Chevalier, Amy; Cousins, Gregory D et al. (2015) Conserved disulfide bond is not essential for the adenosine A2A receptor: Extracellular cysteines influence receptor distribution within the cell and ligand-binding recognition. Biochim Biophys Acta 1848:603-14|
|Guo, Changmiao; Hou, Guangjin; Lu, Xingyu et al. (2014) Fast magic angle spinning NMR with heteronucleus detection for resonance assignments and structural characterization of fully protonated proteins. J Biomol NMR 60:219-29|
|Suiter, Christopher L; Paramasivam, Sivakumar; Hou, Guangjin et al. (2014) Sensitivity gains, linearity, and spectral reproducibility in nonuniformly sampled multidimensional MAS NMR spectra of high dynamic range. J Biomol NMR 59:57-73|
|Lee, Jung-Youn (2014) New and old roles of plasmodesmata in immunity and parallels to tunneling nanotubes. Plant Sci 221-222:13-20|
Showing the most recent 10 out of 47 publications